@article{7c752401d8a9450ebd56a6e7ac77f847,
title = "Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform",
abstract = "Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol \% TiO2 and either 0 mol \% CoO (CoO 0\%) or 2 mol \% CoO (CoO 2\%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.",
keywords = "microfluidics, osteogenic differentiation, phosphate glass, Stem cells, tissue engineering",
author = "Carlotta Peticone and Thompson, \{David De Silva\} and Nikolay Dimov and Ben Jevans and Nick Glass and Martina Micheletti and Knowles, \{Jonathan C.\} and Kim, \{Hae Won\} and Cooper-White, \{Justin J.\} and Wall, \{Ivan B.\}",
note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This work was financially supported by an EPSRC Doctoral Training Grant and Industrial Doctorate Centre in Bioprocess Engineering Leadership (grant number: EP/G034656/1); European Union{\textquoteright}s Horizon 2020 research and innovation programme, under Grant agreement No 739572; National Research Foundation (NRF), Republic of Korea (NRF-2018R1A2B3003446; NRF-2018K1A4A3A01064257). It was also funded in part by the Australian Research Council Discovery Grants Scheme (DP140104217). This work was partly performed at the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and microfabrication facilities for Australia{\textquoteright}s researchers. Funding Information: We gratefully acknowledge Nicolas Szita, Brian Sullivan for access to the microfluidics laboratory at UCL Department of Biochemical Engineering. The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This work was financially supported by an EPSRC Doctoral Training Grant and Industrial Doctorate Centre in Bioprocess Engineering Leadership (grant number: EP/G034656/1); European Union?s Horizon 2020 research and innovation programme, under Grant agreement No 739572; National Research Foundation (NRF), Republic of Korea (NRF-2018R1A2B3003446; NRF-2018K1A4A3A01064257). It was also funded in part by the Australian Research Council Discovery Grants Scheme (DP140104217). This work was partly performed at the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and microfabrication facilities for Australia?s researchers. Publisher Copyright: {\textcopyright} The Author(s) 2020.",
year = "2020",
doi = "10.1177/2041731420954712",
language = "English",
volume = "11",
journal = "Journal of Tissue Engineering (JTE)",
publisher = "SAGE-Hindawi Access to Research",
}