Characterisation of the M918T sodium channel gene mutation associated with strong resistance to pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer).

I. Eleftherianos, S. P. Foster, M. S. Williamson, Ian Denholm

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Recent advances in the characterisation of insect sodium channel gene sequences have identified a small number of point mutations within the channel protein that are implicated in conferring target-site resistance to pyrethroid insecticides (so-called knockdown resistance or kdr). The L1014F (leucine-to-phenylalanine) mutation located in the centre of segment 6 of the domain II region (IIS6) of the sodium channel (the so-called kdr trait) has been detected in the peach-potato aphid, Myzus persicae (Sulzer), and is considered to be the primary cause of pyrethroid resistance in this species. Here we report on the characterisation of a second mutation, M918T (methione-to-threonine), within the nearby IIS4-S5 intracellular linker (the so-called super-kdr trait) in a field clone also possessing L1014F, with both mutations present in heterozygous form. The resistance phenotype of M. persicae clones possessing various combinations of L1014F and M918T to a wide range of pyrethroids (both Type I and II) was assessed in leaf-dip bioassays and to lambda-cyhalothrin applied at up to ten times the recommended field rate as foliar sprays to aphids feeding on whole plants. Bioassay results demonstrated that presence of both mutations was associated with extreme resistance to all the pyrethroids tested relative to aphids lacking the mutations. Furthermore, this resistance well exceeded that shown by aphids that were homozygous for L1014F but lacking M918T. However, pre-treatment with piperonyl butoxide in the leaf-dip bioassays failed to suppress pyrethroid resistance in aphids carrying one or both of the mutations. The relevance of these findings for monitoring and managing pyrethroid resistance in M. persicae populations in the field is discussed
Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalBulletin of entomological research
Volume98
Issue number2
DOIs
Publication statusPublished - 1 Apr 2008

Keywords

  • KNOCKDOWN RESISTANCE
  • HOUSEFLY MUSCA-DOMESTICA
  • SIDE-CHAINS
  • MOLECULAR CHARACTERIZATION
  • pyrethroicis
  • ALCOHOL COMPONENT
  • BLATTELLA-GERMANICA
  • knockdown resistance
  • HEMIPTERA
  • IDENTIFICATION
  • POINT MUTATIONS
  • super-kdr mutation
  • sodium channel
  • Myzus persicae
  • SUPER-KDR

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