TY - JOUR
T1 - Chitosan coated alginate beads for the survival of microencapsulated Lactobacillus plantarum in pomegranate juice
AU - Nualkaekul, Sawaminee
AU - Lenton, Dominique
AU - Cook, Michael T.
AU - Khutoryanskiy, Vitaliy V.
AU - Charalampopoulos, Dimitris
PY - 2012/10/15
Y1 - 2012/10/15
N2 - This work studied the effect of multi-layer coating of alginate beads on the survival of encapsulated Lactobacillus plantarum in simulated gastric solution and during storage in pomegranate juice at 4°C. Uncoated, single and double chitosan coated beads were examined. The survival of the cells in simulated gastric solution (pH 1.5) was improved in the case of the chitosan coated beads by 0.5-2 logs compared to the uncoated beads. The cell concentration in pomegranate juice after six weeks of storage was higher than 5.5 log CFU/mL for single and double coated beads, whereas for free cells and uncoated beads the cells died after 4 weeks of storage. In simulated gastric solution, the size of the beads decreased and their hardness increased with time; however, the opposite trend was observed for pomegranate juice, indicating that there is no correlation between cell survival and the hardness of the beads.
AB - This work studied the effect of multi-layer coating of alginate beads on the survival of encapsulated Lactobacillus plantarum in simulated gastric solution and during storage in pomegranate juice at 4°C. Uncoated, single and double chitosan coated beads were examined. The survival of the cells in simulated gastric solution (pH 1.5) was improved in the case of the chitosan coated beads by 0.5-2 logs compared to the uncoated beads. The cell concentration in pomegranate juice after six weeks of storage was higher than 5.5 log CFU/mL for single and double coated beads, whereas for free cells and uncoated beads the cells died after 4 weeks of storage. In simulated gastric solution, the size of the beads decreased and their hardness increased with time; however, the opposite trend was observed for pomegranate juice, indicating that there is no correlation between cell survival and the hardness of the beads.
KW - Alginate beads
KW - Chitosan
KW - Encapsulation
KW - Lactobacillus plantarum
KW - Multi-layer coating
KW - Pomegranate juice
U2 - 10.1016/j.carbpol.2012.06.073
DO - 10.1016/j.carbpol.2012.06.073
M3 - Article
C2 - 22939342
AN - SCOPUS:84865622875
SN - 0144-8617
VL - 90
SP - 1281
EP - 1287
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
IS - 3
ER -