Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1

P. Hayes; H.J. Meadows; M.J. Gunthorpe; M. Harries; M. Duckworth; W. Cairns; D. C. Harrison; K. Ellington; R. Prinjha; A. Barton; A.D. Medhurst; G.D. Smith; S. Topp; G. Sanger; O. Jenkins; C.D. Benham; I. Gloger; C. E.  Clarke; P. Murdock; J. Terrett; A. D.  Randall; I. S.  Gloger; J. B.  Davis

doi:10.1016/S0304-3959(00)00353-5

Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1

P. Hayes, H.J. Meadows, M.J. Gunthorpe, M. Harries, M. Duckworth, W. Cairns, D. C. Harrison, K. Ellington, R. Prinjha, A. Barton, A.D. Medhurst, G.D. Smith, S. Topp, G. Sanger, O. Jenkins, C.D. Benham, I. Gloger, C. E. Clarke, P. Murdock, J. TerrettA. D. Randall, I. S. Gloger, J. B. Davis

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256 Citations (Scopus)

Abstract

Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1.

Original language	English
Pages (from-to)	205-215
Number of pages	16
Journal	Pain
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3959(00)00353-5
Publication status	Published - 1 Nov 2000

Keywords

Capsaicin
Protons
pH
Temperature
Nociception

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10.1016/S0304-3959(00)00353-5

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Hayes, P., Meadows, H. J., Gunthorpe, M. J., Harries, M., Duckworth, M., Cairns, W., Harrison, D. C., Ellington, K., Prinjha, R., Barton, A., Medhurst, A. D., Smith, G. D., Topp, S., Sanger, G., Jenkins, O., Benham, C. D., Gloger, I., Clarke, C. E., Murdock, P., ... Davis, J. B. (2000). Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1. Pain, 88(2), 205-215. https://doi.org/10.1016/S0304-3959(00)00353-5

@article{3647635600a44fe59d13a647ef305c5f,

title = "Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1",

abstract = "Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1.",

keywords = "Capsaicin, Protons, pH, Temperature, Nociception",

author = "P. Hayes and H.J. Meadows and M.J. Gunthorpe and M. Harries and M. Duckworth and W. Cairns and Harrison, {D. C.} and K. Ellington and R. Prinjha and A. Barton and A.D. Medhurst and G.D. Smith and S. Topp and G. Sanger and O. Jenkins and C.D. Benham and I. Gloger and Clarke, {C. E.} and P. Murdock and J. Terrett and Randall, {A. D.} and Gloger, {I. S.} and Davis, {J. B.}",

note = "Original article can be found at: http://www.sciencedirect.com/science/journal/03043959. Copyright International Association for the Study of Pain.",

year = "2000",

month = nov,

day = "1",

doi = "10.1016/S0304-3959(00)00353-5",

language = "English",

volume = "88",

pages = "205--215",

journal = "Pain",

issn = "0304-3959",

publisher = "Wolters Kluwer",

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Hayes, P, Meadows, HJ, Gunthorpe, MJ, Harries, M, Duckworth, M, Cairns, W, Harrison, DC, Ellington, K, Prinjha, R, Barton, A, Medhurst, AD, Smith, GD, Topp, S, Sanger, G, Jenkins, O, Benham, CD, Gloger, I, Clarke, CE, Murdock, P, Terrett, J, Randall, AD, Gloger, IS & Davis, JB 2000, 'Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1', Pain, vol. 88, no. 2, pp. 205-215. https://doi.org/10.1016/S0304-3959(00)00353-5

TY - JOUR

T1 - Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1

AU - Hayes, P.

AU - Meadows, H.J.

AU - Gunthorpe, M.J.

AU - Harries, M.

AU - Duckworth, M.

AU - Cairns, W.

AU - Harrison, D. C.

AU - Ellington, K.

AU - Prinjha, R.

AU - Barton, A.

AU - Medhurst, A.D.

AU - Smith, G.D.

AU - Topp, S.

AU - Sanger, G.

AU - Jenkins, O.

AU - Benham, C.D.

AU - Gloger, I.

AU - Clarke, C. E.

AU - Murdock, P.

AU - Terrett, J.

AU - Randall, A. D.

AU - Gloger, I. S.

AU - Davis, J. B.

N1 - Original article can be found at: http://www.sciencedirect.com/science/journal/03043959. Copyright International Association for the Study of Pain.

PY - 2000/11/1

Y1 - 2000/11/1

N2 - Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1.

AB - Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1.

KW - Capsaicin

KW - Protons

KW - pH

KW - Temperature

KW - Nociception

U2 - 10.1016/S0304-3959(00)00353-5

DO - 10.1016/S0304-3959(00)00353-5

M3 - Article

SN - 0304-3959

VL - 88

SP - 205

EP - 215

JO - Pain

JF - Pain

IS - 2

ER -

Cloning and functional expression of a human orthologue of the rat vanilloid receptor-1

Abstract

Keywords

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