Abstract
A method is described for the identification of conserved genes in one plant species by using sequence information on internal motifs from well-characterized clones from another species. This sequence information is used to design primers for reverse transcriptase polymerase chain reaction (RT-PCR) and to design oligonucleotide probes to identify genuine positive amplification products. The approach was successfully used to clone cDNAs encoding cold-inducible dehydrin-like genes from the woody perennial blackcurrant,Ribes nigrum L. The strategy described can accelerate the cloning of heterologous cDNAs and is a convenient alternative to direct screening of cDNA libraries
Original language | English |
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Pages (from-to) | 99-102 |
Number of pages | 4 |
Journal | Molecular Biotechnology |
Volume | 7 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 1997 |