Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia

Monika Bokori-Brown, Charlotte Hall, Charlotte Vance, SP Fernandes da Costa, CG Savva, Claire Naylor, AR Cole, AK Basak, DS Moss, Richard Titball

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)
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Abstract

Epsilon toxin (Etx) is a β-pore-forming toxin produced by Clostridium perfringens toxinotypes B and D and plays a key role in the pathogenesis of enterotoxemia, a severe, often fatal disease of ruminants that causes significant economic losses to the farming industry worldwide. This study aimed to determine the potential of a site-directed mutant of Etx (Y30A-Y196A) to be exploited as a recombinant vaccine against enterotoxemia. Replacement of Y30 and Y196 with alanine generated a stable variant of Etx with significantly reduced cell binding and cytotoxic activities in MDCK.2 cells relative to wild type toxin (>430-fold increase in CT50) and Y30A-Y196A was inactive in mice after intraperitoneal administration of trypsin activated toxin at 1000× the expected LD50 dose of trypsin activated wild type toxin. Moreover, polyclonal antibody raised in rabbits against Y30A-Y196A provided protection against wild type toxin in an in vitro neutralisation assay. These data suggest that Y30A-Y196A mutant could form the basis of an improved recombinant vaccine against enterotoxemia.
Original languageEnglish
Pages (from-to)2682-2687
Number of pages6
JournalVaccine
Volume32
Issue number23
Early online date5 Apr 2014
DOIs
Publication statusPublished - 13 May 2014

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