Compact optical trapping microscope using a diode laser

Z. Ulanowski, I.K. Ludlow

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)
    67 Downloads (Pure)

    Abstract

    Single beam gradient force optical trapping (laser tweezers) is increasing in importance as a technique for microparticle and cell manipulation and for the measurement of piconewton forces in aqueous media. An optical trap constructed using an optical microscope and a diode laser is described here. The system incorporates novel beam steering optics allowing the positioning of the trapping spot within the field of view of the microscope. Despite its full functionality the system is compact, portable, relatively inexpensive, and easy to construct and operate. A new scheme for trapping force calibration is also reported and measurements compared to theoretical results. The trapping efficiency is found to be similar to that obtained when using other trapping systems.
    Original languageEnglish
    JournalMeasurement Science and Technology
    Publication statusPublished - 2000

    Keywords

    • optical trapping
    • laser tweezers
    • diode laser
    • trapping force
    • trapping efficiency

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