Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus

Atif Jamal, Elaine M. Bignell, Robert H.A. Coutts

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


A new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in five of 390 A. fumigatus isolates screened. Analysis of purified dsRNA revealed four distinct species 3560, 3159, 3006 and 2863 base pairs in length (dsRNAs 1-4) which were cloned and sequenced. Each dsRNA contains a single open reading frame (ORF) with short 5' and 3' untranslated regions containing strictly conserved termini. The deduced 1114 amino acid (aa) protein (molecular mass=128 kDa) encoded by the dsRNA1 ORF showed homology to the RNA-dependent RNA polymerase (RdRP) of viruses belonging to the Chrysoviridae. Eight motifs characteristic of RdRPs were identified. The dsRNA2 ORF encodes the putative coat protein subunit (953aa; molecular mass=107 kDa). The dsRNA3 and dsRNA4 ORFs respectively encode putative proteins (891aa, molecular mass=99 kDa) and (847aa, molecular mass=95 kDa), both of which have significant similarity to proteins encoded by comparable chrysovirus dsRNAs. The dsRNA profile, amino acid sequence alignments, and phylogenetic analyses all indicate that AfuCV is a new species within the family Chrysoviridae.
Original languageEnglish
Pages (from-to)64-70
Number of pages7
JournalVirus Research
Issue number1
Publication statusPublished - Oct 2010


  • Phylogeny
  • Viral Proteins
  • RNA, Double-Stranded
  • Capsid Proteins
  • Open Reading Frames
  • Amino Acid Sequence
  • Sequence Analysis, DNA
  • Molecular Weight
  • Cloning, Molecular
  • Sequence Alignment
  • Conserved Sequence
  • RNA Viruses
  • Aspergillus fumigatus
  • Molecular Sequence Data
  • 3' Untranslated Regions
  • Sequence Homology, Amino Acid
  • 5' Untranslated Regions
  • Cluster Analysis
  • RNA Replicase


Dive into the research topics of 'Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus'. Together they form a unique fingerprint.

Cite this