Abstract
After initial infection of winter oilseed rape leaves in autumn by air-borne ascospores, Pyrenopeziza brassicae has a long symptomless phase before the first visible necrotic lesions appear, in January/February in the UK or March/April in Poland. Assessment of symptomless P. brassicae infection visually is not reliable before necrotic lesions appear, unless plants are first incubated for
several days at high humidity, in polyethylene bags, to encourage P. brassicae sporulation. Visual methods for assessment of light leaf spot in winter oilseed rape were compared with PCR for detection of P. brassicae infection. PCR diagnosis on leaves sampled from field experiments was more sensitive
than visual assessments, even after incubation, with symptomless infection of leaves detected 2 months earlier by PCR than by incubation in polyethylene bags.
several days at high humidity, in polyethylene bags, to encourage P. brassicae sporulation. Visual methods for assessment of light leaf spot in winter oilseed rape were compared with PCR for detection of P. brassicae infection. PCR diagnosis on leaves sampled from field experiments was more sensitive
than visual assessments, even after incubation, with symptomless infection of leaves detected 2 months earlier by PCR than by incubation in polyethylene bags.
Original language | English |
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Title of host publication | International Organisation for Biological Control Bulletin |
Publisher | IOBC/WPRS |
Pages | 231-236 |
Volume | 29 |
Edition | 7 |
ISBN (Print) | 9290671902 |
Publication status | Published - 2006 |
Event | IOBC-WPRS Working Group: Integrated Control in Oilseed Crops - Poznan, Poland Duration: 11 Oct 2004 → 12 Oct 2004 |
Conference
Conference | IOBC-WPRS Working Group |
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Country/Territory | Poland |
City | Poznan |
Period | 11/10/04 → 12/10/04 |