Detection of Pyrenopeziza brassicae (light leaf spot) infection of winter oilseed rape

Z Karolewski, Bruce Fitt, AO Latunde-Dada, N Evans, SJ Foster

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

After initial infection of winter oilseed rape leaves in autumn by air-borne ascospores, Pyrenopeziza brassicae has a long symptomless phase before the first visible necrotic lesions appear, in January/February in the UK or March/April in Poland. Assessment of symptomless P. brassicae infection visually is not reliable before necrotic lesions appear, unless plants are first incubated for
several days at high humidity, in polyethylene bags, to encourage P. brassicae sporulation. Visual methods for assessment of light leaf spot in winter oilseed rape were compared with PCR for detection of P. brassicae infection. PCR diagnosis on leaves sampled from field experiments was more sensitive
than visual assessments, even after incubation, with symptomless infection of leaves detected 2 months earlier by PCR than by incubation in polyethylene bags.
Original languageEnglish
Title of host publicationInternational Organisation for Biological Control Bulletin
PublisherIOBC/WPRS
Pages231-236
Volume29
Edition7
ISBN (Print)9290671902
Publication statusPublished - 2006
EventIOBC-WPRS Working Group: Integrated Control in Oilseed Crops - Poznan, Poland
Duration: 11 Oct 200412 Oct 2004

Conference

ConferenceIOBC-WPRS Working Group
Country/TerritoryPoland
CityPoznan
Period11/10/0412/10/04

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