Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays

A. P. Van de Wouw; J. F. Stonard; Simon D. Atkins; B. J. Howlett; Jon S. West; Bruce D.L. Fitt

Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays

A. P. Van de Wouw, J. F. Stonard, Simon D. Atkins, B. J. Howlett, Jon S. West, Bruce D.L. Fitt

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Abstract

Control of blackleg (phoma stem canker) disease relies on crop management, fungicides and breeding for disease resistance. Proteins encoded by these resistance genes recognize avirulence (Avr) gene products in the fungus during invasion of the plant. However, these Avr genes can mutate resulting in fungal isolates becoming virulent, thus avoiding recognition by the plant. Widespread use of cultivars containing resistance genes provides strong selection for
isolates that have mutated forms of Avr genes. This selection results in increased frequency of virulent isolates and accordingly, resistance genes become less effective. Molecular tools are now available to assess changes in frequencies of virulent isolates in populations of L. maculans. This paper describes the development of such tools for assessing changes in frequencies of virulent isolates within airborne (ascospore) pathogen inoculum

Original language	English
Title of host publication	16th Australian Research Assembly on Brassicas
Pages	150-153
Publication status	Published - 2009
Event	16th Australian Research Assembly on Brassicas - Ballarat, Australia Duration: 14 Sept 2009 → 16 Sept 2009

Conference

Conference	16th Australian Research Assembly on Brassicas
Country/Territory	Australia
City	Ballarat
Period	14/09/09 → 16/09/09

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http://www.australianoilseeds.com/conferences_workshops/ARAB/ARAB_2009/conference_proceedings

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Van de Wouw, A. P., Stonard, J. F., Atkins, S. D., Howlett, B. J., West, J. S., & Fitt, B. D. L. (2009). Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays. In 16th Australian Research Assembly on Brassicas (pp. 150-153) http://www.australianoilseeds.com/conferences_workshops/ARAB/ARAB_2009/conference_proceedings

@inproceedings{6c12dd9944b4472b89e74a26abdde15f,

title = "Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays",

abstract = "Control of blackleg (phoma stem canker) disease relies on crop management, fungicides and breeding for disease resistance. Proteins encoded by these resistance genes recognize avirulence (Avr) gene products in the fungus during invasion of the plant. However, these Avr genes can mutate resulting in fungal isolates becoming virulent, thus avoiding recognition by the plant. Widespread use of cultivars containing resistance genes provides strong selection for isolates that have mutated forms of Avr genes. This selection results in increased frequency of virulent isolates and accordingly, resistance genes become less effective. Molecular tools are now available to assess changes in frequencies of virulent isolates in populations of L. maculans. This paper describes the development of such tools for assessing changes in frequencies of virulent isolates within airborne (ascospore) pathogen inoculum",

author = "{Van de Wouw}, {A. P.} and Stonard, {J. F.} and Atkins, {Simon D.} and Howlett, {B. J.} and West, {Jon S.} and Fitt, {Bruce D.L.}",

year = "2009",

language = "English",

pages = "150--153",

booktitle = "16th Australian Research Assembly on Brassicas",

note = "16th Australian Research Assembly on Brassicas ; Conference date: 14-09-2009 Through 16-09-2009",

}

Van de Wouw, AP, Stonard, JF, Atkins, SD, Howlett, BJ, West, JS & Fitt, BDL 2009, Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays. in 16th Australian Research Assembly on Brassicas. pp. 150-153, 16th Australian Research Assembly on Brassicas, Ballarat, Australia, 14/09/09. <http://www.australianoilseeds.com/conferences_workshops/ARAB/ARAB_2009/conference_proceedings>

TY - GEN

T1 - Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays

AU - Van de Wouw, A. P.

AU - Stonard, J. F.

AU - Atkins, Simon D.

AU - Howlett, B. J.

AU - West, Jon S.

AU - Fitt, Bruce D.L.

PY - 2009

Y1 - 2009

N2 - Control of blackleg (phoma stem canker) disease relies on crop management, fungicides and breeding for disease resistance. Proteins encoded by these resistance genes recognize avirulence (Avr) gene products in the fungus during invasion of the plant. However, these Avr genes can mutate resulting in fungal isolates becoming virulent, thus avoiding recognition by the plant. Widespread use of cultivars containing resistance genes provides strong selection for isolates that have mutated forms of Avr genes. This selection results in increased frequency of virulent isolates and accordingly, resistance genes become less effective. Molecular tools are now available to assess changes in frequencies of virulent isolates in populations of L. maculans. This paper describes the development of such tools for assessing changes in frequencies of virulent isolates within airborne (ascospore) pathogen inoculum

AB - Control of blackleg (phoma stem canker) disease relies on crop management, fungicides and breeding for disease resistance. Proteins encoded by these resistance genes recognize avirulence (Avr) gene products in the fungus during invasion of the plant. However, these Avr genes can mutate resulting in fungal isolates becoming virulent, thus avoiding recognition by the plant. Widespread use of cultivars containing resistance genes provides strong selection for isolates that have mutated forms of Avr genes. This selection results in increased frequency of virulent isolates and accordingly, resistance genes become less effective. Molecular tools are now available to assess changes in frequencies of virulent isolates in populations of L. maculans. This paper describes the development of such tools for assessing changes in frequencies of virulent isolates within airborne (ascospore) pathogen inoculum

M3 - Conference contribution

SP - 150

EP - 153

BT - 16th Australian Research Assembly on Brassicas

T2 - 16th Australian Research Assembly on Brassicas

Y2 - 14 September 2009 through 16 September 2009

ER -

Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays

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