Development and Application of Molecular Methods for the Study of Polymyxa betae

Euphemia Mutasa-Gottgens; D. M.  Chwarszczynska; G. Williams; E. Ward; M. J. Adams; M. J. C.  Asher

Development and Application of Molecular Methods for the Study of Polymyxa betae

Euphemia Mutasa-Gottgens
, D. M. Chwarszczynska
, G. Williams
, E. Ward
, M. J. Adams
, M. J. C. Asher

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A simple method for cloning Polymyxa betae DNA from infected sugar-beet roots has been developed and used to isolate a multi-copy genomic DNA fragment, pPbKES-1, located on at least four different P. betae chromosomes. This fragment proved to be a good probe for detecting the fungus by Southern/dot blot hybridisation, and a good source of PCR primer sequences for the specific amplification of P.betae DNA. PCR is now used routinely for detecting P.betae in sugar-beet roots and in experiments investigating the epidemiology of the fungus.
Single copy DNA probes have also been isolated and are beginning to provide useful information on variability at the molecular level

Original language	English
Title of host publication	Procs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors
Editors	J.L. Sherwood, C.M. Rush
Publisher	American Society of Sugar Beet Technologists
Pages	173-176
ISBN (Print)	0-926195-02-6
Publication status	Published - 1996
Event	3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors - Dundee, United Kingdom Duration: 6 Aug 1996 → 7 Aug 1996

Conference

Conference	3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors
Country/Territory	United Kingdom
City	Dundee
Period	6/08/96 → 7/08/96

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http://amarillo.tamu.edu/files/2010/12/1996-IWGPVFV-Proceedings3.pdf

Cite this

Mutasa-Gottgens, E., Chwarszczynska, D. M., Williams, G., Ward, E., Adams, M. J., & Asher, M. J. C. (1996). Development and Application of Molecular Methods for the Study of Polymyxa betae. In J. L. Sherwood, & C. M. Rush (Eds.), Procs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors (pp. 173-176). American Society of Sugar Beet Technologists. http://amarillo.tamu.edu/files/2010/12/1996-IWGPVFV-Proceedings3.pdf

@inproceedings{317852e6c7174d7eb44c432562dd732a,

title = "Development and Application of Molecular Methods for the Study of Polymyxa betae",

abstract = "A simple method for cloning Polymyxa betae DNA from infected sugar-beet roots has been developed and used to isolate a multi-copy genomic DNA fragment, pPbKES-1, located on at least four different P. betae chromosomes. This fragment proved to be a good probe for detecting the fungus by Southern/dot blot hybridisation, and a good source of PCR primer sequences for the specific amplification of P.betae DNA. PCR is now used routinely for detecting P.betae in sugar-beet roots and in experiments investigating the epidemiology of the fungus. Single copy DNA probes have also been isolated and are beginning to provide useful information on variability at the molecular level",

author = "Euphemia Mutasa-Gottgens and Chwarszczynska, \{D. M.\} and G. Williams and E. Ward and Adams, \{M. J.\} and Asher, \{M. J. C.\}",

year = "1996",

language = "English",

isbn = "0-926195-02-6",

pages = "173--176",

editor = "J.L. Sherwood and C.M. Rush",

booktitle = "Procs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors",

publisher = "American Society of Sugar Beet Technologists",

note = "3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors ; Conference date: 06-08-1996 Through 07-08-1996",

}

Mutasa-Gottgens, E, Chwarszczynska, DM, Williams, G, Ward, E, Adams, MJ & Asher, MJC 1996, Development and Application of Molecular Methods for the Study of Polymyxa betae. in JL Sherwood & CM Rush (eds), Procs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors. American Society of Sugar Beet Technologists, pp. 173-176, 3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors, Dundee, United Kingdom, 6/08/96. <http://amarillo.tamu.edu/files/2010/12/1996-IWGPVFV-Proceedings3.pdf>

Development and Application of Molecular Methods for the Study of Polymyxa betae. / Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M. ; Williams, G. et al.
Procs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors. ed. / J.L. Sherwood; C.M. Rush. American Society of Sugar Beet Technologists, 1996. p. 173-176.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Development and Application of Molecular Methods for the Study of Polymyxa betae

AU - Mutasa-Gottgens, Euphemia

AU - Chwarszczynska, D. M.

AU - Williams, G.

AU - Ward, E.

AU - Adams, M. J.

AU - Asher, M. J. C.

PY - 1996

Y1 - 1996

N2 - A simple method for cloning Polymyxa betae DNA from infected sugar-beet roots has been developed and used to isolate a multi-copy genomic DNA fragment, pPbKES-1, located on at least four different P. betae chromosomes. This fragment proved to be a good probe for detecting the fungus by Southern/dot blot hybridisation, and a good source of PCR primer sequences for the specific amplification of P.betae DNA. PCR is now used routinely for detecting P.betae in sugar-beet roots and in experiments investigating the epidemiology of the fungus. Single copy DNA probes have also been isolated and are beginning to provide useful information on variability at the molecular level

AB - A simple method for cloning Polymyxa betae DNA from infected sugar-beet roots has been developed and used to isolate a multi-copy genomic DNA fragment, pPbKES-1, located on at least four different P. betae chromosomes. This fragment proved to be a good probe for detecting the fungus by Southern/dot blot hybridisation, and a good source of PCR primer sequences for the specific amplification of P.betae DNA. PCR is now used routinely for detecting P.betae in sugar-beet roots and in experiments investigating the epidemiology of the fungus. Single copy DNA probes have also been isolated and are beginning to provide useful information on variability at the molecular level

M3 - Conference contribution

SN - 0-926195-02-6

SP - 173

EP - 176

BT - Procs 3rd Symposium of the Int. Working Group on Plant Viruses with Fungal Vectors

A2 - Sherwood, J.L.

A2 - Rush, C.M.

PB - American Society of Sugar Beet Technologists

T2 - 3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors

Y2 - 6 August 1996 through 7 August 1996

ER -

Development and Application of Molecular Methods for the Study of Polymyxa betae

Abstract

Conference

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