Development and validation of a HPTLC method for the estimation of cefuroxime axetil

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the determination of cefuroxime axetil in dosage form. The stationary phase used was precoated silica gel 60F ₂₅₄ . The mobile phase used was a mixture of chloroform:methanol:toluene (4:2:2 v/v). The detection of spot was carried out at 290 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 300 to 800 ng/spot for cefuroxime axetil. The limit of detection and the limit of quantification for the cefuroxime axetil were found to be 50 ng/spot and 100 ng/spot. The proposed method can be successfully used to determine the drug content of marketed formulation.