Abstract
A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.
Original language | English |
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Pages (from-to) | 1032-1035 |
Number of pages | 4 |
Journal | Plant Pathology |
Volume | 56 |
Issue number | 6 |
DOIs | |
Publication status | Published - Dec 2007 |
Keywords
- DOUBLE-STRANDED RNAS
- amasya cherry disease
- dsRNAs
- cherry trees
- Prunus avium
- diagnosis
- CHLOROTIC RUSTY SPOT
- COMPLEX PATTERN