Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

Z. Kozlakidis, A. Citir, S. Acikgoz, Robert H.A. Coutts

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.

Original languageEnglish
Pages (from-to)1032-1035
Number of pages4
JournalPlant Pathology
Volume56
Issue number6
DOIs
Publication statusPublished - Dec 2007

Keywords

  • DOUBLE-STRANDED RNAS
  • amasya cherry disease
  • dsRNAs
  • cherry trees
  • Prunus avium
  • diagnosis
  • CHLOROTIC RUSTY SPOT
  • COMPLEX PATTERN

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