Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

Z. Kozlakidis; A. Citir; S. Acikgoz; Robert H.A. Coutts

doi:10.1111/j.1365-3059.2007.01690.x

Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

Z. Kozlakidis
, A. Citir
, S. Acikgoz
, Robert H.A. Coutts

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.

Original language	English
Pages (from-to)	1032-1035
Number of pages	4
Journal	Plant Pathology
Volume	56
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-3059.2007.01690.x
Publication status	Published - Dec 2007

Keywords

DOUBLE-STRANDED RNAS
amasya cherry disease
dsRNAs
cherry trees
Prunus avium
diagnosis
CHLOROTIC RUSTY SPOT
COMPLEX PATTERN

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10.1111/j.1365-3059.2007.01690.x

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title = "Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease",

abstract = "A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.",

keywords = "DOUBLE-STRANDED RNAS, amasya cherry disease, dsRNAs, cherry trees, Prunus avium, diagnosis, CHLOROTIC RUSTY SPOT, COMPLEX PATTERN",

author = "Z. Kozlakidis and A. Citir and S. Acikgoz and Coutts, \{Robert H.A.\}",

year = "2007",

month = dec,

doi = "10.1111/j.1365-3059.2007.01690.x",

language = "English",

volume = "56",

pages = "1032--1035",

journal = "Plant Pathology",

issn = "0032-0862",

publisher = "Wiley-Blackwell",

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T1 - Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

AU - Kozlakidis, Z.

AU - Citir, A.

AU - Acikgoz, S.

AU - Coutts, Robert H.A.

PY - 2007/12

Y1 - 2007/12

N2 - A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.

AB - A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.

KW - DOUBLE-STRANDED RNAS

KW - amasya cherry disease

KW - dsRNAs

KW - cherry trees

KW - Prunus avium

KW - diagnosis

KW - CHLOROTIC RUSTY SPOT

KW - COMPLEX PATTERN

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DO - 10.1111/j.1365-3059.2007.01690.x

M3 - Article

SN - 0032-0862

VL - 56

SP - 1032

EP - 1035

JO - Plant Pathology

JF - Plant Pathology

IS - 6

ER -

Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

Abstract

Keywords

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