TY - JOUR
T1 - Development of PCR for the detection of Polymyxa-betae in sugar beet roots and its application in field studies
AU - Mutasa-Gottgens, Euphemia
AU - Chwarszczynska, D. M.
AU - Adams, Michael J.
AU - Ward, Elaine
AU - Asher, M. J. C.
PY - 1995/11
Y1 - 1995/11
N2 - A previously cloned 1.8 kb PCR Polymyxa betae DNA fragment has been fully sequenced and used to design PCR primers for the specific detection of P. betae in sugar beet seedling roots. The primers did not amplify sequences from fungi closely related to P. betae, or from sugar beet or other microorganisms commonly associated wit h sugar beet roots. Protocols which combine PCR with Southern/dot blot analysis of products were developed to detect P. betae in sugar beet roots. A nested PCR protocol was also developed and shown to provide levels of sensitivity that allow more rapid screening for P. betae infection. Subsequently, PCR was used to detect P. betae in a survey of infection incidence in 65 sugar beet Fields, Seventy one percent of fields and 33% of plants sampled were infected by late June, with a lower incidence on peat as compared to mineral soils
AB - A previously cloned 1.8 kb PCR Polymyxa betae DNA fragment has been fully sequenced and used to design PCR primers for the specific detection of P. betae in sugar beet seedling roots. The primers did not amplify sequences from fungi closely related to P. betae, or from sugar beet or other microorganisms commonly associated wit h sugar beet roots. Protocols which combine PCR with Southern/dot blot analysis of products were developed to detect P. betae in sugar beet roots. A nested PCR protocol was also developed and shown to provide levels of sensitivity that allow more rapid screening for P. betae infection. Subsequently, PCR was used to detect P. betae in a survey of infection incidence in 65 sugar beet Fields, Seventy one percent of fields and 33% of plants sampled were infected by late June, with a lower incidence on peat as compared to mineral soils
U2 - 10.1006/pmpp.1995.1060
DO - 10.1006/pmpp.1995.1060
M3 - Article
SN - 0885-5765
VL - 47
SP - 303
EP - 313
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
IS - 5
ER -