Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells

Elizabeth Haythorne, Maria Rohm, Martijn van de Bunt, Melissa F Brereton, Andrei I Tarasov, Thomas S Blacker, Gregor Sachse, Mariana Silva Dos Santos, Raul Terron Exposito, Simon Davis, Otto Baba, Roman Fischer, Michael R Duchen, Patrik Rorsman, James I MacRae, Frances M Ashcroft

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64 Citations (Scopus)
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Abstract

Diabetes is a global health problem caused primarily by the inability of pancreatic β-cells to secrete adequate levels of insulin. The molecular mechanisms underlying the progressive failure of β-cells to respond to glucose in type-2 diabetes remain unresolved. Using a combination of transcriptomics and proteomics, we find significant dysregulation of major metabolic pathways in islets of diabetic βV59M mice, a non-obese, eulipidaemic diabetes model. Multiple genes/proteins involved in glycolysis/gluconeogenesis are upregulated, whereas those involved in oxidative phosphorylation are downregulated. In isolated islets, glucose-induced increases in NADH and ATP are impaired and both oxidative and glycolytic glucose metabolism are reduced. INS-1 β-cells cultured chronically at high glucose show similar changes in protein expression and reduced glucose-stimulated oxygen consumption: targeted metabolomics reveals impaired metabolism. These data indicate hyperglycaemia induces metabolic changes in β-cells that markedly reduce mitochondrial metabolism and ATP synthesis. We propose this underlies the progressive failure of β-cells in diabetes.
Original languageEnglish
Article number2474
Number of pages17
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 6 Jun 2019

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