Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons

S. Kulshreshtha, Robert H.A. Coutts

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

An in vitro protocol has been developed for direct somatic embryogenesis of zygotic cotyledons from mature sugarbeet (Beta vulgaris L.) embryos. Explants were sequentially cultured on modified Murashige and Skoog (MS) medium supplemented with different combinations of 2,4-D, NAA, BAP and TIBA. Somatic embryogenesis was induced within 4 weeks of culture on embryogenesis induction medium which contained MS medium supplemented with BAP and TIBA. Proliferation of somatic embryos was observed on embryo proliferation medium, which contained MS medium supplemented with BAP and NAA within 4 weeks of culture. Plants were regenerated on hormone free 1/4 strength MS medium containing a low sucrose concentration. With some sugarbeet lines, high frequencies of plant regeneration in excess of 90% were observed. The incorporation of TIBA in the media was essential for successful regeneration.
Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of Plant Growth Regulation
Volume22
Issue number2
DOIs
Publication statusPublished - 1997

Fingerprint

Dive into the research topics of 'Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons'. Together they form a unique fingerprint.

Cite this