TY - JOUR
T1 - Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons
AU - Kulshreshtha, S.
AU - Coutts, Robert H.A.
PY - 1997
Y1 - 1997
N2 - An in vitro protocol has been developed for direct somatic embryogenesis of zygotic cotyledons from mature sugarbeet (Beta vulgaris L.) embryos. Explants were sequentially cultured on modified Murashige and Skoog (MS) medium supplemented with different combinations of 2,4-D, NAA, BAP and TIBA. Somatic embryogenesis was induced within 4 weeks of culture on embryogenesis induction medium which contained MS medium supplemented with BAP and TIBA. Proliferation of somatic embryos was observed on embryo proliferation medium, which contained MS medium supplemented with BAP and NAA within 4 weeks of culture. Plants were regenerated on hormone free 1/4 strength MS medium containing a low sucrose concentration. With some sugarbeet lines, high frequencies of plant regeneration in excess of 90% were observed. The incorporation of TIBA in the media was essential for successful regeneration.
AB - An in vitro protocol has been developed for direct somatic embryogenesis of zygotic cotyledons from mature sugarbeet (Beta vulgaris L.) embryos. Explants were sequentially cultured on modified Murashige and Skoog (MS) medium supplemented with different combinations of 2,4-D, NAA, BAP and TIBA. Somatic embryogenesis was induced within 4 weeks of culture on embryogenesis induction medium which contained MS medium supplemented with BAP and TIBA. Proliferation of somatic embryos was observed on embryo proliferation medium, which contained MS medium supplemented with BAP and NAA within 4 weeks of culture. Plants were regenerated on hormone free 1/4 strength MS medium containing a low sucrose concentration. With some sugarbeet lines, high frequencies of plant regeneration in excess of 90% were observed. The incorporation of TIBA in the media was essential for successful regeneration.
UR - http://www.scopus.com/inward/record.url?scp=0030862091&partnerID=8YFLogxK
U2 - 10.1023/A:1005889429586
DO - 10.1023/A:1005889429586
M3 - Article
AN - SCOPUS:0030862091
SN - 0167-6903
VL - 22
SP - 87
EP - 92
JO - Journal of Plant Growth Regulation
JF - Journal of Plant Growth Regulation
IS - 2
ER -