DNA polymerase V-dependent mutator activity in an SOS-induced Escherichia coli strain with a temperature-sensitive DNA polymerase III

Andrew R Timms, Bryn A Bridges

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The temperature-sensitive DNA polymerase III (Pol III) encoded by the dnaE486 allele confers a spontaneous mutator activity in SOS-induced bacteria that is largely dependent upon DNA polymerase V (Pol V), encoded by umuD, C. This mutator activity is influenced by the defective proof-reading sub-unit of Pol III encoded by the dnaQ905 (mutD5) allele arguing that Pol V is most likely fixing mutations arising from mismatched primer termini produced by Pol III(486). The size of the dnaQ effect is, however, modest leaving open the possibility that Pol V may be responsible for some of the mutator effect by engaging in bursts of processive activity.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalMutation research
Volume499
Issue number1
DOIs
Publication statusPublished - 29 Jan 2002

Keywords

  • DNA Polymerase III/genetics
  • DNA-Directed DNA Polymerase/physiology
  • Escherichia coli/physiology
  • Escherichia coli Proteins/genetics
  • Exodeoxyribonuclease V
  • Exodeoxyribonucleases/genetics
  • Mutation
  • SOS Response (Genetics)
  • Temperature

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