Dysregulated placental microRNAs in Early and Late onset Preeclampsia

Alexandra Lykoudi; Aggeliki Kolialexi; George I Lambrou; Maria Braoudaki; Charalampos Siristatidis; George Konstantinos Papaioanou; Maria Tzetis; Ariadni Mavrou; Nikolas Papantoniou

doi:10.1016/j.placenta.2017.11.005

Dysregulated placental microRNAs in Early and Late onset Preeclampsia

Alexandra Lykoudi, Aggeliki Kolialexi, George I Lambrou, Maria Braoudaki, Charalampos Siristatidis, George Konstantinos Papaioanou, Maria Tzetis, Ariadni Mavrou, Nikolas Papantoniou

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Abstract

INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies.

METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction.

RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR.

DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.

Original language	English
Pages (from-to)	24-32
Number of pages	9
Journal	Placenta
Volume	61
Early online date	14 Nov 2017
DOIs	https://doi.org/10.1016/j.placenta.2017.11.005
Publication status	Published - 1 Jan 2018

Keywords

microRNAs
preeclampsia

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10.1016/j.placenta.2017.11.005Licence: Other

PlacentaAccepted author manuscript, 571 KBLicence: CC BY-NC-ND

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title = "Dysregulated placental microRNAs in Early and Late onset Preeclampsia",

abstract = "INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies.METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB{\textregistered} simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction.RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR.DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.",

keywords = "microRNAs, preeclampsia",

author = "Alexandra Lykoudi and Aggeliki Kolialexi and Lambrou, {George I} and Maria Braoudaki and Charalampos Siristatidis and Papaioanou, {George Konstantinos} and Maria Tzetis and Ariadni Mavrou and Nikolas Papantoniou",

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T1 - Dysregulated placental microRNAs in Early and Late onset Preeclampsia

AU - Lykoudi, Alexandra

AU - Kolialexi, Aggeliki

AU - Lambrou, George I

AU - Braoudaki, Maria

AU - Siristatidis, Charalampos

AU - Papaioanou, George Konstantinos

AU - Tzetis, Maria

AU - Mavrou, Ariadni

AU - Papantoniou, Nikolas

PY - 2018/1/1

Y1 - 2018/1/1

N2 - INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies.METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction.RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR.DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.

AB - INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies.METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction.RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR.DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.

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KW - preeclampsia

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DO - 10.1016/j.placenta.2017.11.005

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SP - 24

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JO - Placenta

JF - Placenta

ER -

Dysregulated placental microRNAs in Early and Late onset Preeclampsia

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