Effect of ethnicity, gender and age on the amount and composition of residual skin surface components derived from sebum, sweat and epidermal lipids

Satyajit Shetage, M.J. Traynor, Marc Brown, Mahad Raji, Diepiriyie Graham-Kalio, Robert Chilcott

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)
295 Downloads (Pure)

Abstract

Background/purpose: The superficial layer on the skin surface, known as the acid mantle, comprises a mixture of sebum, sweat, corneocyte debris and constituents of natural moisturizing factor. Thus, the phrase 'residual skin surface components' (RSSC) is an appropriate term for the mixture of substances recovered from the skin surface. There is no general agreement about the effects of ethnicity, gender and age on RSSC. The aim of this human volunteer study was to evaluate RSSC in relation to ethnicity, gender and age. A suitable acquisition medium for RSSC collection was identified and samples of RSSC were subsequently analysed using gas chromatography-mass spectrometry (GC-MS) and gravimetry. Methods: A total of 315 volunteers participated in the study from a range of self-declared ethnic backgrounds. Six acquisition media were compared to determine the most suitable media for RSSC collection. The effect of age, gender and ethnicity on RSSC collection was evaluated by gravimetric analysis while GC-MS was used to determine the composition of RSSC. Results: Of the six candidate materials assessed, cigarette paper provided the most practical and reproducible sample acquisition medium. There was no significant difference in the amount of RSSC collected when based on gender and ethnicity and no significant correlation between RSSC recovery and age. Up to 49 compounds were detected from human RSSC when analysed by GC-MS. Conclusions: The results of the present study suggest that RSSC can be effectively collected using cigarette paper and analysed by GC-MS. Ethnicity, gender and age had no significant impact on the quantity of RSSC recovered from the skin surface.
Original languageEnglish
Pages (from-to)97-107
Number of pages11
JournalSkin Research and Technology
Volume20
Issue number1
Early online date19 Jul 2013
DOIs
Publication statusPublished - Feb 2014

Keywords

  • sebum
  • skin surface lipids
  • cigarette paper
  • ethnicity
  • gender
  • age
  • gas chromatography-mass spectrometry

Fingerprint

Dive into the research topics of 'Effect of ethnicity, gender and age on the amount and composition of residual skin surface components derived from sebum, sweat and epidermal lipids'. Together they form a unique fingerprint.

Cite this