The first aim of the present study was to quantify and compare asymmetries among professional soccer and cricket athletes. The second aim was to examine the association between asymmetries and performance within both groups. Professional soccer (n = 18) and cricket (n = 23) athletes performed single leg countermovement jumps (SLCMJ), single leg drop jumps (SLDJ), a 10 m sprint and 505 change of direction speed (CODS) tests. Inter-limb asymmetries were calculated as a standard percentage difference, Mann Whitney U tests conducted to establish systematic bias between groups and Spearmans r correlations used to establish the relationship between asymmetry scores and speed and CODS performance. Soccer athletes sprinted faster, jumped higher and had a greater reactive strength index (RSI) score than cricket athletes (p < 0.05). However, cricketers showed reduced ground contact times compared to footballers during the SLDJ (p < 0.05). The cricket group showed significantly greater jump height (asymmetry = 11.49 vs. 6.51%; p = 0.015) and RSI (asymmetry = 10.37 vs. 5.95%; p = 0.014) asymmetries compared to soccer players. These metrics were also associated with slower 505 times in the cricket group only (r = 0.56 to 0.74; p < 0.01). These results show that between-limb asymmetries exhibit no association with speed and CODS in elite soccer players, but are associated with reduced CODS in elite cricketers. Thus, the reduction of inter-limb asymmetries may be of greater consideration when working with cricket versus soccer athletes.