Abstract
The effects of nanoparticles of CuO on delayed rectifier potassium current (Ik) were studied in acutely isolated CA1 pyramidal neurons of rat hippocampus by using the whole-cell patch-clamp techniques and the effects on differentiated PC12 cell viability were studied with MTT assay. Nanoparticles of CuO mainly inhibited Ik in the concentration of 5×10－5 g/mL. Nanoparticles of CuO didn't shift the steady-state activation curve of Ik but negatively shift the inactivation curve of Ik. Nanoparticles of CuO (5×10－4, 5×10－5 and 5×10－6 g/mL) induced differentiated PC12 cells damage with dose-and time-dependent manner.
| Original language | English |
|---|---|
| Pages (from-to) | 123-128 |
| Number of pages | 6 |
| Journal | Acta Biophysica Sinica |
| Volume | 24 |
| Issue number | 2 |
| Publication status | Published - 2008 |
Keywords
- nanomaterial
- delayed rectifier potassium
- hippocampal neurons
- PC12
- biological effects