Electromagnetic Actuated Stiring in Microbioreactor Enabling Easier Multiplexing and Flexible Device Design

M. J. Davies, Ian Munro, Christabel Tan, M.C. Tracey, Nicolas Szita

Research output: Contribution to conferencePaperpeer-review

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Abstract

The development of a novel electromagnetically (EM) actuated stirring method, for use in microbioreactors, is reported. Mixing in microbioreactors is critical to ensure even distribution of nutrients to microorganisms and cells. Magnetically driven stirrer bars or peristaltic mixing are the most commonly utilised mixing methods employed in completely liquid-filled microbioreactors. However the circular reactor shape required for mixing with a stirrer bar and frequently used for peristaltically mixed microbioreactors presents difficulties for bubble-free priming in a microfluidic bioreactor. Moreover the circular shape and the
hardware required for both types of mixing reduces the potential packing density of multiplexed reactors. We present a new method of mixing, displaying design flexibility by demonstrating mixing in circular and diamond-shaped reactors and a duplex diamond reactor and fermentation of the gram-positive bacteria S. carnosus in a diamond-shaped microbioreactor system. The results of the optimisation of this mixing method for performing fermentations alongside both batch and continuous culture fermentations are presented
Original languageEnglish
Publication statusPublished - 7 Sept 2014
Event4th Micro and Nano Flows Conference - UCL, London, United Kingdom
Duration: 7 Sept 201410 Sept 2014

Conference

Conference4th Micro and Nano Flows Conference
Country/TerritoryUnited Kingdom
CityLondon
Period7/09/1410/09/14

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