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Abstract

Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the electrowetting effect. The presence of charges results in an electrically induced spreading of the droplet which permits purposeful manipulation across a hydrophobic surface. Here, we demonstrate EWOD-based protocol for sample processing and detection of four categories of antigens, using an automated surface actuation platform, via two variations of an Enzyme-Linked Immunosorbent Assay (ELISA) methods. The ELISA is performed on magnetic beads with immobilized primary antibodies which can be selected to target a specific antigen. An antibody conjugated to HRP binds to the antigen and is mixed with H 2O 2/Luminol for quantification of the captured pathogens. Assay completion times of between 6 and 10 min were achieved, whilst minuscule volumes of reagents were utilized.

Original languageEnglish
Article numbere60489
JournalJournal of Visualized Experiments
Volume2020
Issue number156
DOIs
Publication statusPublished - 23 Feb 2020

Keywords

  • Automation
  • Biodetection
  • Bioengineering
  • Chemiluminescence
  • DMF
  • Digital microfluidics
  • ELISA
  • EWOD
  • Electrowetting on Dielectric
  • Enzyme-Linked Immunosorbent Assay
  • Issue 156
  • Pathogen quantification

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