Elemental fingerprinting of Hypericum perforatum (St John's Wort) herb and preparations using ICP-OES and chemometrics

Jade D. Owen, Stewart B. Kirton, Sara J. Evans, Jacqueline L. Stair

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17 Citations (Scopus)
84 Downloads (Pure)

Abstract

St. John's wort (SJW) (Hypericum perforatum) is a herbal remedy commonly used to treat mild depression. The elemental profiles of 54 samples (i.e., dry herbs, tablets and capsules) were evaluated by monitoring 25 elements using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The major elemental constituents in the SJW samples were Ca (300-199,000 μg/g), Mg (410-3,530 μg/g), Al (4.4-900 μg/g), Fe (1.154-760 μg/g), Mn (2.4-261 μg/g), Sr (0.88-83.6 μg/g), and Zn (7-64 μg/g). For the sixteen elements that could be reliably quantified, principal component analysis (PCA) was used to investigate underlying patterns in the data. PCA models identified 7 key elements (i.e., Ba, Ca, Cd, Mg, Mo, Ni and Y), which described 85% of the variance in the dataset in the first three principal components. The PCA approach resulted in a general delineation between the three different formulations and provides a basis for monitoring product quality in this manner.
Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume125
Early online date4 Mar 2016
DOIs
Publication statusPublished - 5 Jun 2016

Keywords

  • Hypericum perforatum
  • ICP-OES
  • Metal content
  • Principal component analysis
  • St. John's wort

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