Endospore heterogeneity in Pasteuria-Penetrans related to adhesion to plant-parasitic nematodes

Keith Davies; M. Redden; T. K.  Pearson

doi:10.1111/j.1472-765X.1994.tb00478.x

Endospore heterogeneity in Pasteuria-Penetrans related to adhesion to plant-parasitic nematodes

Keith Davies, M. Redden, T. K. Pearson

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Abstract

Hybridoma cell lines were screened by indirect immunofluorescence for the secretion of monoclonal antibodies (Mabs) to the surface of a population of endospores of the obligate nematode hyperparasite Pasteuria penetrans. Whereas polyclonal antibodies from test sera samples recognized 100% of the spores, five selected Mabs recognized different proportions of the spores ranging from 10 to 90% showing that the spore population was highly heterogeneous. Probing spores adhering to different nematode populations with the five Mabs showed that different subpopulations of the spores were specific to different nematode populations indicating cuticular heterogeneity among the nematode populations. Each of the five Mabs recognized a far larger proportion of the spores adhering to the nematode population on which the Pasteuria population was originally cultured than of those adhering to other populations.

Original language	English
Pages (from-to)	370-373
Number of pages	4
Journal	Letters in Applied Microbiology
Volume	19
Issue number	5
DOIs	https://doi.org/10.1111/j.1472-765X.1994.tb00478.x
Publication status	Published - Nov 1994

Keywords

MELOIDOGYNE-INCOGNITA
SP-NOV
HYPERPARASITE
HOST

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10.1111/j.1472-765X.1994.tb00478.x

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title = "Endospore heterogeneity in Pasteuria-Penetrans related to adhesion to plant-parasitic nematodes",

abstract = "Hybridoma cell lines were screened by indirect immunofluorescence for the secretion of monoclonal antibodies (Mabs) to the surface of a population of endospores of the obligate nematode hyperparasite Pasteuria penetrans. Whereas polyclonal antibodies from test sera samples recognized 100% of the spores, five selected Mabs recognized different proportions of the spores ranging from 10 to 90% showing that the spore population was highly heterogeneous. Probing spores adhering to different nematode populations with the five Mabs showed that different subpopulations of the spores were specific to different nematode populations indicating cuticular heterogeneity among the nematode populations. Each of the five Mabs recognized a far larger proportion of the spores adhering to the nematode population on which the Pasteuria population was originally cultured than of those adhering to other populations.",

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T1 - Endospore heterogeneity in Pasteuria-Penetrans related to adhesion to plant-parasitic nematodes

AU - Davies, Keith

AU - Redden, M.

AU - Pearson, T. K.

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Y1 - 1994/11

N2 - Hybridoma cell lines were screened by indirect immunofluorescence for the secretion of monoclonal antibodies (Mabs) to the surface of a population of endospores of the obligate nematode hyperparasite Pasteuria penetrans. Whereas polyclonal antibodies from test sera samples recognized 100% of the spores, five selected Mabs recognized different proportions of the spores ranging from 10 to 90% showing that the spore population was highly heterogeneous. Probing spores adhering to different nematode populations with the five Mabs showed that different subpopulations of the spores were specific to different nematode populations indicating cuticular heterogeneity among the nematode populations. Each of the five Mabs recognized a far larger proportion of the spores adhering to the nematode population on which the Pasteuria population was originally cultured than of those adhering to other populations.

AB - Hybridoma cell lines were screened by indirect immunofluorescence for the secretion of monoclonal antibodies (Mabs) to the surface of a population of endospores of the obligate nematode hyperparasite Pasteuria penetrans. Whereas polyclonal antibodies from test sera samples recognized 100% of the spores, five selected Mabs recognized different proportions of the spores ranging from 10 to 90% showing that the spore population was highly heterogeneous. Probing spores adhering to different nematode populations with the five Mabs showed that different subpopulations of the spores were specific to different nematode populations indicating cuticular heterogeneity among the nematode populations. Each of the five Mabs recognized a far larger proportion of the spores adhering to the nematode population on which the Pasteuria population was originally cultured than of those adhering to other populations.

KW - MELOIDOGYNE-INCOGNITA

KW - SP-NOV

KW - HYPERPARASITE

KW - HOST

U2 - 10.1111/j.1472-765X.1994.tb00478.x

DO - 10.1111/j.1472-765X.1994.tb00478.x

M3 - Article

SN - 0266-8254

VL - 19

SP - 370

EP - 373

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 5

ER -

Endospore heterogeneity in Pasteuria-Penetrans related to adhesion to plant-parasitic nematodes

Abstract

Keywords

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