Twenty different strains of Pectobacterium carotovorum subsp. carotovorum (Pcc) wererecovered using plant host enrichment approach from soil of different vegetables growing fields of Gujarat, India, during 2006-9. These strains have been classified into five different biovars by differences in physiological and biochemical features, and identified as Pcc by species-specific PCR and 16s rDNA sequences. Moreover Pcc strains were clearly differentiated based on virulence trait, plant cell wall degrading enzymes production and phylogenetic analysis of 16s rDNA sequence and Repetitive extragenic palindromic- PCR (rep-PCR). rep-PCR typing using four different primer sets revealed a high genetic variability amongst these strains independent from their pathogenic nature, with some of them showing identical banding pattern although isolated from different soils and years. The variability among isolated strains indicates that factors other than plant host correlate to genetic variability during host-pathogen interaction of this economic important Pcc strains. Based on these polyphasic characterizations of Pcc strainsisolated from soil have revealed heterogeneity among them, can be considered as useful studies for epidemiological surveillance (or distribution) of soft rot causing Pcc in semi arid region of India.
|Publication status||In preparation - 2014|