Abstract
The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity
Original language | English |
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Pages (from-to) | 77-81 |
Number of pages | 5 |
Journal | Journal of Cellular Physiology |
Volume | 215 |
Issue number | 1 |
Early online date | 24 Oct 2007 |
DOIs | |
Publication status | Published - Apr 2008 |