Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity.

Yanning Ding; William J. Brackenbury; Pinar U. Onganer; Ximena Montano; Louise M. Porter; Lucy F. Bates; Mustafa B.A. Djamgoz

doi:10.1002/jcp.21289

Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity.

Yanning Ding, William J. Brackenbury, Pinar U. Onganer, Ximena Montano, Louise M. Porter, Lucy F. Bates, Mustafa B.A. Djamgoz

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity

Original language	English
Pages (from-to)	77-81
Number of pages	5
Journal	Journal of Cellular Physiology
Volume	215
Issue number	1
Early online date	24 Oct 2007
DOIs	https://doi.org/10.1002/jcp.21289
Publication status	Published - Apr 2008

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title = "Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity.",

abstract = "The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65\% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity",

author = "Yanning Ding and Brackenbury, \{William J.\} and Onganer, \{Pinar U.\} and Ximena Montano and Porter, \{Louise M.\} and Bates, \{Lucy F.\} and Djamgoz, \{Mustafa B.A.\}",

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T1 - Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity.

AU - Ding, Yanning

AU - Brackenbury, William J.

AU - Onganer, Pinar U.

AU - Montano, Ximena

AU - Porter, Louise M.

AU - Bates, Lucy F.

AU - Djamgoz, Mustafa B.A.

PY - 2008/4

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N2 - The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity

AB - The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity

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SN - 1097-4652

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JO - Journal of Cellular Physiology

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ER -

Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity.

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