TY - JOUR
T1 - Evaluation of genotoxic and apoptotic potential of Hypericum adenotrichum Spach. in vitro
AU - Sarimahmut, Mehmet
AU - Balikci, Necmiye
AU - Celikler, Serap
AU - Ari, Ferda
AU - Ulukaya, Engin
AU - Guleryuz, Gurcan
AU - Ozel, Mustafa Zafer
N1 - Funding Information:
We thank the Research Fund of Uludag University for the project numbered UAP(F)-2009/28 for providing us with the kits/chemicals. The authors would like to thank Özgür Vatan, Ph.D. for technical assistance, Selvi Durmuş Erim, Ph.D. for her comments on linguistic improvement and American Journal Experts for editing the manuscript.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Hypericum adenotrichum Spach. is an endemic plant from Turkey that is also used in folk medicine. In this study, following analyses of its chemical composition, the genotoxic/antigenotoxic effects of the methanol extract of H. adenotrichum in human lymphocyte culture were investigated using in vitro sister chromatid exchange, micronucleus and comet assays. In addition, the anti-growth effect of the extract was investigated in human breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT and ATP viability assays. The mode of cell death was determined using fluorescence microscopy and biochemical methods. We found that the H. adenotrichum extract demonstrated cytotoxic and genotoxic effects in a cell type-dependent manner. At selected doses (125-500 μg/ml), the H. adenotrichum extract exhibited significant genotoxic activity in human lymphocytes, whereas it showed anti-growth effects on cancer cell lines between 0.2 and 100 μg/ml concentrations. The mode of cell death in cancer cells was shown to be apoptosis due to the presence of pyknotic nuclei, the cleavage of poly-(ADP-ribose) polymerase (PARP) and/or the activation of caspase-3. These results suggest that H. adenotrichum might show both cytotoxic and genotoxic effects depending on the cell type. This should be taken into account in its use for therapeutic purposes.
AB - Hypericum adenotrichum Spach. is an endemic plant from Turkey that is also used in folk medicine. In this study, following analyses of its chemical composition, the genotoxic/antigenotoxic effects of the methanol extract of H. adenotrichum in human lymphocyte culture were investigated using in vitro sister chromatid exchange, micronucleus and comet assays. In addition, the anti-growth effect of the extract was investigated in human breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT and ATP viability assays. The mode of cell death was determined using fluorescence microscopy and biochemical methods. We found that the H. adenotrichum extract demonstrated cytotoxic and genotoxic effects in a cell type-dependent manner. At selected doses (125-500 μg/ml), the H. adenotrichum extract exhibited significant genotoxic activity in human lymphocytes, whereas it showed anti-growth effects on cancer cell lines between 0.2 and 100 μg/ml concentrations. The mode of cell death in cancer cells was shown to be apoptosis due to the presence of pyknotic nuclei, the cleavage of poly-(ADP-ribose) polymerase (PARP) and/or the activation of caspase-3. These results suggest that H. adenotrichum might show both cytotoxic and genotoxic effects depending on the cell type. This should be taken into account in its use for therapeutic purposes.
KW - Apoptosis
KW - Breast cancer
KW - Genotoxicity
KW - Hypericum adenotrichum
UR - http://www.scopus.com/inward/record.url?scp=84953205974&partnerID=8YFLogxK
U2 - 10.1016/j.yrtph.2015.11.010
DO - 10.1016/j.yrtph.2015.11.010
M3 - Article
C2 - 26617407
AN - SCOPUS:84953205974
SN - 0273-2300
VL - 74
SP - 137
EP - 146
JO - Regulatory Toxicology and Pharmacology
JF - Regulatory Toxicology and Pharmacology
ER -