Evaluation of the rat airway epithelial RL-65 cell lin as a novel in vitro model for screening inhaled drug candidates

Victoria Hutter; Natasha Tang; Vanessa Zann; Anne Cooper; David Pritchard; Cynthia Bosquillon

Evaluation of the rat airway epithelial RL-65 cell lin as a novel in vitro model for screening inhaled drug candidates

Victoria Hutter, Natasha Tang, Vanessa Zann, Anne Cooper, David Pritchard, Cynthia Bosquillon

Research output: Contribution to conference › Abstract › peer-review

Abstract

The rat airway epithelial cell line RL65 was assessed for its potential as an in-vitro permeability screening tool of inhaled compounds. Cells were grown on Transwell® cell culture supports at an air-liquid interface in serum-free medium. Cell layers exhibited transepithelial electrical resistance (TEER) values >200Ω.cm2 after 14 days in culture when seeded at a density of 1 x 104 cells/cm2. A coefficient of apparent permeability (Papp) ~2.5 x 10-6 cm/s was obtained for the paracellular markers, fluorescein sodium and 14C-mannitol. No vectorial transport was observed for the drug transporter substrates rhodamine 123 and 3H-digoxin.

Original language	English
Pages	1230-1232
Number of pages	1
Publication status	Published - 2010
Event	UK Pharm Sci 2010 - Nottingham, United Kingdom Duration: 1 Sept 2010 → 3 Sept 2010

Conference

Conference	UK Pharm Sci 2010
Country/Territory	United Kingdom
City	Nottingham
Period	1/09/10 → 3/09/10

Cite this

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title = "Evaluation of the rat airway epithelial RL-65 cell lin as a novel in vitro model for screening inhaled drug candidates",

abstract = "The rat airway epithelial cell line RL65 was assessed for its potential as an in-vitro permeability screening tool of inhaled compounds. Cells were grown on Transwell{\textregistered} cell culture supports at an air-liquid interface in serum-free medium. Cell layers exhibited transepithelial electrical resistance (TEER) values >200Ω.cm2 after 14 days in culture when seeded at a density of 1 x 104 cells/cm2. A coefficient of apparent permeability (Papp) ~2.5 x 10-6 cm/s was obtained for the paracellular markers, fluorescein sodium and 14C-mannitol. No vectorial transport was observed for the drug transporter substrates rhodamine 123 and 3H-digoxin.",

author = "Victoria Hutter and Natasha Tang and Vanessa Zann and Anne Cooper and David Pritchard and Cynthia Bosquillon",

year = "2010",

language = "English",

pages = "1230--1232",

note = "UK Pharm Sci 2010 ; Conference date: 01-09-2010 Through 03-09-2010",

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TY - CONF

T1 - Evaluation of the rat airway epithelial RL-65 cell lin as a novel in vitro model for screening inhaled drug candidates

AU - Hutter, Victoria

AU - Tang, Natasha

AU - Zann, Vanessa

AU - Cooper, Anne

AU - Pritchard, David

AU - Bosquillon, Cynthia

PY - 2010

Y1 - 2010

N2 - The rat airway epithelial cell line RL65 was assessed for its potential as an in-vitro permeability screening tool of inhaled compounds. Cells were grown on Transwell® cell culture supports at an air-liquid interface in serum-free medium. Cell layers exhibited transepithelial electrical resistance (TEER) values >200Ω.cm2 after 14 days in culture when seeded at a density of 1 x 104 cells/cm2. A coefficient of apparent permeability (Papp) ~2.5 x 10-6 cm/s was obtained for the paracellular markers, fluorescein sodium and 14C-mannitol. No vectorial transport was observed for the drug transporter substrates rhodamine 123 and 3H-digoxin.

AB - The rat airway epithelial cell line RL65 was assessed for its potential as an in-vitro permeability screening tool of inhaled compounds. Cells were grown on Transwell® cell culture supports at an air-liquid interface in serum-free medium. Cell layers exhibited transepithelial electrical resistance (TEER) values >200Ω.cm2 after 14 days in culture when seeded at a density of 1 x 104 cells/cm2. A coefficient of apparent permeability (Papp) ~2.5 x 10-6 cm/s was obtained for the paracellular markers, fluorescein sodium and 14C-mannitol. No vectorial transport was observed for the drug transporter substrates rhodamine 123 and 3H-digoxin.

M3 - Abstract

SP - 1230

EP - 1232

T2 - UK Pharm Sci 2010

Y2 - 1 September 2010 through 3 September 2010

ER -

Evaluation of the rat airway epithelial RL-65 cell lin as a novel in vitro model for screening inhaled drug candidates

Abstract

Conference

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