Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons

Ioly Kotta-Loizou; Ioannis Karakasiliotis; Niki Vassilaki; Panagiotis Sakellariou; Ralf Bartenschlager; Penelope Mavromara

doi:10.1128/JVI.02351-14

Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons

Ioly Kotta-Loizou, Ioannis Karakasiliotis, Niki Vassilaki, Panagiotis Sakellariou, Ralf Bartenschlager, Penelope Mavromara

School of Life and Medical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

Original language	English
Pages (from-to)	5164-70
Number of pages	7
Journal	Journal of Virology
Volume	89
Issue number	9
DOIs	https://doi.org/10.1128/JVI.02351-14
Publication status	Published - May 2015

Keywords

Cell Line
Gene Expression
Gene Expression Profiling
Hepacivirus/genetics
Hepatocytes/virology
Humans
Protein Isoforms/biosynthesis
Replicon
Viral Core Proteins/biosynthesis

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10.1128/JVI.02351-14

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title = "Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons",

abstract = "Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.",

keywords = "Cell Line, Gene Expression, Gene Expression Profiling, Hepacivirus/genetics, Hepatocytes/virology, Humans, Protein Isoforms/biosynthesis, Replicon, Viral Core Proteins/biosynthesis",

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AU - Kotta-Loizou, Ioly

AU - Karakasiliotis, Ioannis

AU - Vassilaki, Niki

AU - Sakellariou, Panagiotis

AU - Bartenschlager, Ralf

AU - Mavromara, Penelope

PY - 2015/5

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N2 - Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

AB - Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

KW - Cell Line

KW - Gene Expression

KW - Gene Expression Profiling

KW - Hepacivirus/genetics

KW - Hepatocytes/virology

KW - Humans

KW - Protein Isoforms/biosynthesis

KW - Replicon

KW - Viral Core Proteins/biosynthesis

U2 - 10.1128/JVI.02351-14

DO - 10.1128/JVI.02351-14

M3 - Article

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VL - 89

SP - 5164

EP - 5170

JO - Journal of Virology

JF - Journal of Virology

IS - 9

ER -

Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons

Abstract

Keywords

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