Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil

Alessandra Marcon Gasperini; Esther Garcia-Cela; Michael Sulyok; A. Medina; Naresh Magan

doi:10.1007/s12550-020-00414-8

Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil

Alessandra Marcon Gasperini, Esther Garcia-Cela, Michael Sulyok, A. Medina, Naresh Magan

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Abstract

There is little knowledge of the microbial diversity, mycotoxins and associated secondary metabolites in GM maize and isogenic non-GM cultivars (cvs). This study has quantified the microbial populations and dominant fungal genera in 6 cvs of each type representative of herbicide, pesticide or stacked resistance to both. The predominant mycotoxins and targeted metabolomics profiles were also compared between the two sets of cvs. This showed that the overall fungal populations were 8.8 CFUs g−1 maize. The dominant genera, isolated from maize samples, whether surface-sterilised or not, in all maize cvs were Fusarium, followed by Penicillium, Aspergillus and occasionally Cladosporium and Alternaria. The analysis of the targeted metabolomics showed that approx. 29 different metabolites were detected. These were dominated by fumonisins and minor Penicillium spp. metabolites (questiomycin A and rugulovasine A). Interestingly, the range and number of mycotoxins present in the GM cvs were significantly lower than in the non-GM maize samples. This suggests that while the fungal diversity of the two types of maize appeared to be very similar, the major contaminant mycotoxins and range of toxic secondary metabolites were much lower in the GM cvs.

Original language	English
Pages (from-to)	39 - 48
Number of pages	10
Journal	Mycotoxin Research
Volume	37
Issue number	1
Early online date	12 Oct 2020
DOIs	https://doi.org/10.1007/s12550-020-00414-8 https://doi.org/10.1007/s12550-020-00414-8
Publication status	Published - 1 Feb 2021

Keywords

Aflatoxin
maize
metabolomics
Metabolomics
Maize cultivars
Selective media
GM
Toxic secondary metabolites
Mycotoxigenic fungi
Non-GM
Fungal diversity

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The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, http://creativecommons.org/licenses/by/4.0/.
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abstract = "There is little knowledge of the microbial diversity, mycotoxins and associated secondary metabolites in GM maize and isogenic non-GM cultivars (cvs). This study has quantified the microbial populations and dominant fungal genera in 6 cvs of each type representative of herbicide, pesticide or stacked resistance to both. The predominant mycotoxins and targeted metabolomics profiles were also compared between the two sets of cvs. This showed that the overall fungal populations were 8.8 CFUs g−1 maize. The dominant genera, isolated from maize samples, whether surface-sterilised or not, in all maize cvs were Fusarium, followed by Penicillium, Aspergillus and occasionally Cladosporium and Alternaria. The analysis of the targeted metabolomics showed that approx. 29 different metabolites were detected. These were dominated by fumonisins and minor Penicillium spp. metabolites (questiomycin A and rugulovasine A). Interestingly, the range and number of mycotoxins present in the GM cvs were significantly lower than in the non-GM maize samples. This suggests that while the fungal diversity of the two types of maize appeared to be very similar, the major contaminant mycotoxins and range of toxic secondary metabolites were much lower in the GM cvs.",

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Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil

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Keywords

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