Guidelines for the Use of Cell Lines in Biomedical Research

R. Geraghty, A. Capes-Davis, John Davis, J. Downward, R.I. Freshney, I. Knezevic, R. Lovell-Badge, J.R.W. Masters, J. Meredith, G.N. Stacey, P. Thraves, M. Vias

Research output: Contribution to journalReview articlepeer-review

187 Citations (Scopus)


Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic
and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the
necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching
and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them
effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines
between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on
complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection
and maintenance of equipment and how to deal with problems that may arise.
Original languageEnglish
Pages (from-to)1021-1046
Number of pages26
JournalBritish Journal of Cancer (BJC)
Publication statusPublished - 2014


  • cell culture
  • best practice
  • contamination
  • misidentification
  • cryopresevation


Dive into the research topics of 'Guidelines for the Use of Cell Lines in Biomedical Research'. Together they form a unique fingerprint.

Cite this