Human papillomavirus is detected in transitional cell carcinoma arising in renal transplant recipients

Ehab Husain, David M. Prowse, Elena Ktori, Taufiq Shaikh, Magdi Yaqoob, Islam Junaid, Suhail Baithun

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
179 Downloads (Pure)

Abstract

Aims: We investigated the role of human papillomavirus HPV in the development of transitional cell carcinoma TCC arising in renal transplant recipients. Methods: Genomic DNA was extracted from 10 m paraffin embedded sections of five TCCs arising in five renal transplant recipients using the QIAamp DNA mini kit according to the manufacturer's instructions. β-globin PCR was performed to test DNA adequacy. Samples were tested for the presence of HPV DNA by broad spectrum HPV PCR method using non-biotinylated SPF10 primers SPF1A, SPF1B, SPF1C, SPF1D, SPF2B, SPF2D which amplify a short 65 bp fragment. Positive bands were identified on a 3 gel. Positive samples underwent a second HPV PCR and were amplified using biotinylated SPF10 primer set, which amplifies the same 65 bp region of the L1 open reading frame. INNO-LiPA line probe assay was then performed to genotype the samples which uses a reverse hybridisation principle. Results: Four of five TCCs examined were positive for HPV. The high risk HPV16 was detected in three cases whereas in the fourth case an unclassifiable HPV genotype was present. In all DNA samples, β-globin amplification was successful. Conclusions: Our results indicate that HPV and in particular HPV16 may play an aetiological role in the development of TCC in renal transplant patients.
Original languageEnglish
Pages (from-to)245-247
Number of pages3
JournalPathology
Volume41
Issue number3
DOIs
Publication statusPublished - 9 Nov 2009

Keywords

  • Carcinogenesis
  • HPV
  • Immunosuppression
  • Renal transplant
  • TCC

Fingerprint

Dive into the research topics of 'Human papillomavirus is detected in transitional cell carcinoma arising in renal transplant recipients'. Together they form a unique fingerprint.

Cite this