Identification and molecular characterization of rose leaf curl virus in ornamental pomegranate (Punica granatum L.)

Kamran Rashid; Mohsin Tariq; Rob W. Briddon; Ioly Kotta-Loizou; Khadim Hussain

doi:10.1007/s13313-021-00780-6

Identification and molecular characterization of rose leaf curl virus in ornamental pomegranate (Punica granatum L.)

Kamran Rashid, Mohsin Tariq, Rob W. Briddon, Ioly Kotta-Loizou, Khadim Hussain

Research output: Contribution to journal › Article › peer-review

Abstract

Symptoms of leaf curling and yellowing, typical of begomoviruses, were observed on ornamental pomegranate plants in a park in Faisalabad, Pakistan. Leaves from symptomatic plants were collected and DNA extracted. PCR amplification with universal primers for the amplification of begomoviruses amplified an approx. 2.8 kb fragment which was cloned and sequenced. Similar amplifications with primers for begomovirus DNA B component and betasatellites were uniformly negative and no amplifications were obtained with DNA extracted from a non-symptomatic pomegranate plant. Sequence comparisons showed the cloned fragment to be an isolate of rose leaf curl virus (RoLCuV). This is the first time RoLCuV has been identified in a bushy shrub pomegranate. Sequence analysis shows the RoLCuV isolate from pomegranate to have a recombination history distinct from other RoLCV isolates.

Original language	English
Pages (from-to)	353-356
Number of pages	4
Journal	Australasian Plant Pathology
Volume	50
Issue number	3
DOIs	https://doi.org/10.1007/s13313-021-00780-6
Publication status	Published - May 2021
Externally published	Yes

Keywords

Begomovirus
Geminivirus
Perennial plants
Pomegranate
Rose leaf curl virus

Access to Document

10.1007/s13313-021-00780-6

Cite this

@article{b5761ca9ea8749d6be5f6df8545e5996,

title = "Identification and molecular characterization of rose leaf curl virus in ornamental pomegranate (Punica granatum L.)",

abstract = "Symptoms of leaf curling and yellowing, typical of begomoviruses, were observed on ornamental pomegranate plants in a park in Faisalabad, Pakistan. Leaves from symptomatic plants were collected and DNA extracted. PCR amplification with universal primers for the amplification of begomoviruses amplified an approx. 2.8 kb fragment which was cloned and sequenced. Similar amplifications with primers for begomovirus DNA B component and betasatellites were uniformly negative and no amplifications were obtained with DNA extracted from a non-symptomatic pomegranate plant. Sequence comparisons showed the cloned fragment to be an isolate of rose leaf curl virus (RoLCuV). This is the first time RoLCuV has been identified in a bushy shrub pomegranate. Sequence analysis shows the RoLCuV isolate from pomegranate to have a recombination history distinct from other RoLCV isolates.",

keywords = "Begomovirus, Geminivirus, Perennial plants, Pomegranate, Rose leaf curl virus",

author = "Kamran Rashid and Mohsin Tariq and Briddon, {Rob W.} and Ioly Kotta-Loizou and Khadim Hussain",

note = "Publisher Copyright: {\textcopyright} 2021, Australasian Plant Pathology Society Inc.",

year = "2021",

month = may,

doi = "10.1007/s13313-021-00780-6",

language = "English",

volume = "50",

pages = "353--356",

journal = "Australasian Plant Pathology",

issn = "0815-3191",

publisher = "Springer Nature Link",

number = "3",

}

TY - JOUR

T1 - Identification and molecular characterization of rose leaf curl virus in ornamental pomegranate (Punica granatum L.)

AU - Rashid, Kamran

AU - Tariq, Mohsin

AU - Briddon, Rob W.

AU - Kotta-Loizou, Ioly

AU - Hussain, Khadim

PY - 2021/5

Y1 - 2021/5

N2 - Symptoms of leaf curling and yellowing, typical of begomoviruses, were observed on ornamental pomegranate plants in a park in Faisalabad, Pakistan. Leaves from symptomatic plants were collected and DNA extracted. PCR amplification with universal primers for the amplification of begomoviruses amplified an approx. 2.8 kb fragment which was cloned and sequenced. Similar amplifications with primers for begomovirus DNA B component and betasatellites were uniformly negative and no amplifications were obtained with DNA extracted from a non-symptomatic pomegranate plant. Sequence comparisons showed the cloned fragment to be an isolate of rose leaf curl virus (RoLCuV). This is the first time RoLCuV has been identified in a bushy shrub pomegranate. Sequence analysis shows the RoLCuV isolate from pomegranate to have a recombination history distinct from other RoLCV isolates.

AB - Symptoms of leaf curling and yellowing, typical of begomoviruses, were observed on ornamental pomegranate plants in a park in Faisalabad, Pakistan. Leaves from symptomatic plants were collected and DNA extracted. PCR amplification with universal primers for the amplification of begomoviruses amplified an approx. 2.8 kb fragment which was cloned and sequenced. Similar amplifications with primers for begomovirus DNA B component and betasatellites were uniformly negative and no amplifications were obtained with DNA extracted from a non-symptomatic pomegranate plant. Sequence comparisons showed the cloned fragment to be an isolate of rose leaf curl virus (RoLCuV). This is the first time RoLCuV has been identified in a bushy shrub pomegranate. Sequence analysis shows the RoLCuV isolate from pomegranate to have a recombination history distinct from other RoLCV isolates.

KW - Begomovirus

KW - Geminivirus

KW - Perennial plants

KW - Pomegranate

KW - Rose leaf curl virus

UR - http://www.scopus.com/inward/record.url?scp=85101776629&partnerID=8YFLogxK

U2 - 10.1007/s13313-021-00780-6

DO - 10.1007/s13313-021-00780-6

M3 - Article

AN - SCOPUS:85101776629

SN - 0815-3191

VL - 50

SP - 353

EP - 356

JO - Australasian Plant Pathology

JF - Australasian Plant Pathology

IS - 3

ER -

Identification and molecular characterization of rose leaf curl virus in ornamental pomegranate (Punica granatum L.)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this