TY - JOUR
T1 - Identification and sequence determination of a new chrysovirus infecting the phytopathogenic fungus Dothistroma septosporum
AU - Shah, Unnati A.
AU - Daudu, John O.
AU - Filippou, Charalampos
AU - Tubby, Katherine V.
AU - Coutts, Robert H. A.
AU - Kotta-Loizou, Ioly
N1 - © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, to view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
PY - 2023/4/18
Y1 - 2023/4/18
N2 - A new double-stranded (ds) RNA mycovirus has been identified in isolate Ds752-1 of the phytopathogenic fungus Dothistroma septosporum, the causal agent of Dothistroma needle blight, also known as red band needle blight or pine needle blight. Dothistroma septosporum chrysovirus 1 (DsCV-1) is a new member of the genus Alphachrysovirus in the family Chrysoviridae. The DsCV-1 genome comprises four dsRNA elements designated 1, 2, 3, and 4 from largest to smallest. dsRNA1 encodes an RNA-dependent RNA polymerase (RdRP) that is most similar to the RdRP of Erysiphe necator associated chrysovirus 3. dsRNA2 potentially encodes two hypothetical proteins, one of which is small and has no homology to known proteins, and one of which is large with significant sequence similarity to the alphachryso-P3 of other alphachrysoviruses. dsRNA3 and dsRNA4 encode a coat protein (CP) and a putative cysteine protease, respectively. This is the first report of a mycovirus infecting the fungus D. septosporum, and DsCV-1 is one of three Chrysoviridae family members found to possess genomic dsRNAs potentially encoding more than one protein.
AB - A new double-stranded (ds) RNA mycovirus has been identified in isolate Ds752-1 of the phytopathogenic fungus Dothistroma septosporum, the causal agent of Dothistroma needle blight, also known as red band needle blight or pine needle blight. Dothistroma septosporum chrysovirus 1 (DsCV-1) is a new member of the genus Alphachrysovirus in the family Chrysoviridae. The DsCV-1 genome comprises four dsRNA elements designated 1, 2, 3, and 4 from largest to smallest. dsRNA1 encodes an RNA-dependent RNA polymerase (RdRP) that is most similar to the RdRP of Erysiphe necator associated chrysovirus 3. dsRNA2 potentially encodes two hypothetical proteins, one of which is small and has no homology to known proteins, and one of which is large with significant sequence similarity to the alphachryso-P3 of other alphachrysoviruses. dsRNA3 and dsRNA4 encode a coat protein (CP) and a putative cysteine protease, respectively. This is the first report of a mycovirus infecting the fungus D. septosporum, and DsCV-1 is one of three Chrysoviridae family members found to possess genomic dsRNAs potentially encoding more than one protein.
KW - Annotated Sequence Record
KW - Genome, Viral
KW - RNA-Dependent RNA Polymerase/genetics
KW - Fungal Viruses/genetics
KW - Viral Proteins/genetics
KW - Open Reading Frames
KW - RNA, Viral/genetics
KW - RNA Viruses
KW - Ascomycota/genetics
KW - RNA, Double-Stranded/genetics
KW - Phylogeny
KW - Sequence Analysis, DNA
UR - http://www.scopus.com/inward/record.url?scp=85152975302&partnerID=8YFLogxK
U2 - 10.1007/s00705-023-05768-9
DO - 10.1007/s00705-023-05768-9
M3 - Article
C2 - 37071213
SN - 0304-8608
VL - 168
SP - 1
EP - 5
JO - Archives of Virology
JF - Archives of Virology
IS - 5
M1 - 144
ER -