Identification of a New Membrane-associated Polypeptide Specified by the Coronavirus Infectious Bronchitis Virus

Allan Smith, M. E. G. Boursnell, M. M. Binns, T. D. K. Brown, S. C. Inglis

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with β-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.
    Original languageEnglish
    Pages (from-to)3-11
    JournalJournal of General Virology
    Volume71
    DOIs
    Publication statusPublished - 1990

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