Identification of membrane proteins of human blood platelets with a hydrophobic photolabel

Theo Berkhout, M. E. Schiphorst, K. W. Wirtz, J. J. Sixma

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2 Citations (Scopus)


A photoactivable glycolipid probe, 12-(4-azido-2-nitrophenoxy)stearoyl[1-14C]glucosamine, was used to label proteins and lipids of platelet membranes. The proteins were analyzed by two-dimensional high-resolution gelelectrophoresis. The labeling patterns showed that three membrane proteins were labeled which were not previously identified by ectolabeling (Sixma, J.J. and Schiphorst, M.E. (1980) Biochim. Biophys. Acta 603, 70-83). Analysis of the lipid fraction showed that phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine were labeled by the probe. The distinct labeling of phosphatidylserine strongly suggests that the probe redistributes between the two halves of the bilayer.

Original languageEnglish
Pages (from-to)298-304
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number2
Publication statusPublished - 5 Dec 1984


  • Azides
  • Blood Platelets
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Membrane Proteins
  • Phospholipids
  • Photochemistry


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