Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

Shan Goh; Daniel Ngugi; Regina Lizundia; Isabel Hostettler; Kerry Woods; Keith Ballingall; Niall D MacHugh; W Ivan Morrison; Willie Weir; Brian Shiels; Dirk Werling

doi:10.1371/journal.pone.0162571

Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

Shan Goh
, Daniel Ngugi
, Regina Lizundia
, Isabel Hostettler
, Kerry Woods
, Keith Ballingall
, Niall D MacHugh
, W Ivan Morrison
, Willie Weir
, Brian Shiels
, Dirk Werling

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

45 Downloads (Pure)

Abstract

As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.

Original language	English
Pages (from-to)	e0162571
Journal	PLoS ONE
Volume	11
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0162571
Publication status	Published - 9 Sept 2016

Keywords

Journal Article

Access to Document

10.1371/journal.pone.0162571Licence: CC BY

Published VersionFinal published version, 1.73 MBLicence: CC BY

Cite this

@article{6d7d16923e3745639cb2bdc9302ce4e9,

title = "Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells",

abstract = "As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.",

keywords = "Journal Article",

author = "Shan Goh and Daniel Ngugi and Regina Lizundia and Isabel Hostettler and Kerry Woods and Keith Ballingall and MacHugh, \{Niall D\} and Morrison, \{W Ivan\} and Willie Weir and Brian Shiels and Dirk Werling",

note = "This is an open access article distributed under the terms of the Creative Commons Attribution License, CC BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2016",

month = sep,

day = "9",

doi = "10.1371/journal.pone.0162571",

language = "English",

volume = "11",

pages = "e0162571",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "9",

}

TY - JOUR

T1 - Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

AU - Goh, Shan

AU - Ngugi, Daniel

AU - Lizundia, Regina

AU - Hostettler, Isabel

AU - Woods, Kerry

AU - Ballingall, Keith

AU - MacHugh, Niall D

AU - Morrison, W Ivan

AU - Weir, Willie

AU - Shiels, Brian

AU - Werling, Dirk

N1 - This is an open access article distributed under the terms of the Creative Commons Attribution License, CC BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2016/9/9

Y1 - 2016/9/9

N2 - As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.

AB - As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.

KW - Journal Article

U2 - 10.1371/journal.pone.0162571

DO - 10.1371/journal.pone.0162571

M3 - Article

C2 - 27611868

SN - 1932-6203

VL - 11

SP - e0162571

JO - PLoS ONE

JF - PLoS ONE

IS - 9

ER -

Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this