Imaging of X-Ray-Excited Emissions from Quantum Dots and Biological Tissue in Whole Mouse

Sean G. Ryan, Matthew N. Butler, Segun Adeyemi, Tammy Kalber, Peter Stephen Patrick, May Zaw Thin, Ian F. Harrison, Daniel J. Stuckey, Martin Pule, Mark Lythgoe

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
52 Downloads (Pure)

Abstract

Optical imaging in clinical and preclinical settings can provide a wealth of biological information, particularly when coupled with targetted nanoparticles, but optical scattering and absorption limit the depth and resolution in both animal and human subjects. Two new hybrid approaches are presented, using the penetrating power of X-rays to increase the depth of optical imaging. Foremost, we demonstrate the excitation by X-rays of quantum-dots (QD) emitting in the near-infrared (NIR), using a clinical X-ray system to map the distribution of QDs at depth in whole mouse. We elicit a clear, spatially-resolved NIR signal from deep organs (brain, liver and kidney) with short (1 second) exposures and tolerable radiation doses that will permit future in vivo applications. Furthermore, X-ray-excited endogenous emission is also detected from whole mouse. The use of keV X-rays to excite emission from QDs and tissue represent novel biomedical imaging technologies, and exploit emerging QDs as optical probes for spatial-temporal molecular imaging at greater depth than previously possible.
Original languageEnglish
Article number19223
Number of pages10
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 16 Dec 2019

Keywords

  • X-ray imaging
  • quantum dots
  • nanoparticles
  • biosensors
  • nanobiotechnology
  • optical imaging

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