Immunocytochemical localization of the elongation factor Tu in E. coli cells

M. Schilstra, J.W. Slot, P.H. Van der Meide, G. Posthuma, A.F.M. Cremers, L. Bosch

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    14 Citations (Scopus)


    The localization of the elongation factor Tu (EF-Tu) in ultrathin cryosections of E. coli cells was determined with the electron microscope using a highly specific immunological labellin technique. EF-Tu is distributed almost homogeneously throughout the cytoplasm. Although it has often been suggested that EF-Tu could be part of a putative prokaryotic cytoskeleton, we did not find any evidence for supramolecular assemblies, such as fibres or filaments, containing a large amount of EF-Tu. EF-Tu was not observed in association with the outer cell membrane and periplasmic space. A topological relationship with the inner membrane is not apparent in our micrographs. In cells in which the EF-Tu level is raised significantly, the protein piles up in discrete cell regions.
    Original languageEnglish
    Pages (from-to)175-179
    JournalFEBS Letters
    Issue number2
    Publication statusPublished - 1984


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