In vitro colonic metabolism of coffee and chlorogenic acid results in selective changes in human faecal microbiota growth

Charlotte E. Mills, Xenofon Tzounis, Maria Jose Oruna-Concha, Don S. Mottram, Glenn R. Gibson, Jeremy P E Spencer

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Coffee is a relatively rich source of chlorogenic acids (CGA), which, as other polyphenols, have been postulated to exert preventive effects against CVD and type 2 diabetes. As a considerable proportion of ingested CGA reaches the large intestine, CGA may be capable of exerting beneficial effects in the large gut. Here, we utilise a stirred, anaerobic, pH-controlled, batch culture fermentation model of the distal region of the colon in order to investigate the impact of coffee and CGA on the growth of the human faecal microbiota. Incubation of coffee samples with the human faecal microbiota led to the rapid metabolism of CGA (4' h) and the production of dihydrocaffeic acid and dihydroferulic acid, while caffeine remained unmetabolised. The coffee with the highest levels of CGA (P<' 0·05, relative to the other coffees) induced a significant increase in the growth of Bifidobacterium spp. relative to the control vessel at 10' h after exposure (P<' 0·05). Similarly, an equivalent quantity of CGA (80·8' mg, matched with that in high-CGA coffee) induced a significant increase in the growth of Bifidobacterium spp. (P<' 0·05). CGA alone also induced a significant increase in the growth of the Clostridium coccoides-Eubacterium rectale group (P<' 0·05). This selective metabolism and subsequent amplification of specific bacterial populations could be beneficial to host health.

Original languageEnglish
Pages (from-to)1220-1227
Number of pages8
JournalBritish Journal of Nutrition
Volume113
Issue number8
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Chlorogenic acids
  • Coffee (Coffea spp.)
  • Colonic microbiota

Fingerprint

Dive into the research topics of 'In vitro colonic metabolism of coffee and chlorogenic acid results in selective changes in human faecal microbiota growth'. Together they form a unique fingerprint.

Cite this