Inhibitory effect of tungsten carbide nanoparticles on voltage-gated potassium currents of hippocampal CA1 neurons

Dehong Shan; Yongling Xie; Guogang Ren; Zhuo Yang

doi:10.1016/j.toxlet.2011.12.001

Inhibitory effect of tungsten carbide nanoparticles on voltage-gated potassium currents of hippocampal CA1 neurons

Dehong Shan, Yongling Xie, Guogang Ren, Zhuo Yang

University of Hertfordshire

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The effects of tungsten carbide nanoparticles (nano-WC) on the properties of voltage-dependent potassium currents and evoked action potentials were studied in the hippocampal CA1 pyramidal neurons of rats at the ages of postnatal days 10-14 using the whole-cell patch-clamp technique. The results indicated that: (1) the amplitudes of transient outward potassium current (I _A) and delayed rectifier potassium current (I _K) were significantly decreased by 10 ^-7g/ml nano-WC, while the current-voltage curves of I _A and I _K were significantly decreased by nano-WC from +10 to +90mV. (2) Nano-WC produced a depolarizing shift in the steady-state activation curve of I _A and I _K with increased slope factors, and delayed the recovery of I _A from inactivation, but no significant effects were found on the inactivation of I _A. (3) Nano-WC prolonged the evoked action potential duration and lowered the firing rate. These results suggest that 10 ^-7g/ml nano-WC can decrease the amplitudes of I _A and I _K currents by reducing the opening number of voltage-gated potassium channels and delaying the recovery of I _A from inactivation, which indicate that nano-WC has the potential neurotoxicity.

Original language	English
Pages (from-to)	129-135
Number of pages	7
Journal	Toxicology Letters
Volume	209
Issue number	2
Early online date	13 Dec 2011
DOIs	https://doi.org/10.1016/j.toxlet.2011.12.001
Publication status	Published - 7 Mar 2012

Keywords

Delayed rectifier potassium current
Hippocampus
Neurotoxicity
Transient outward potassium current
Tungsten carbide nanoparticles

Access to Document

10.1016/j.toxlet.2011.12.001

Cite this

@article{1d919c86267e4c3d956422c6fa4d9f1a,

title = "Inhibitory effect of tungsten carbide nanoparticles on voltage-gated potassium currents of hippocampal CA1 neurons",

abstract = "The effects of tungsten carbide nanoparticles (nano-WC) on the properties of voltage-dependent potassium currents and evoked action potentials were studied in the hippocampal CA1 pyramidal neurons of rats at the ages of postnatal days 10-14 using the whole-cell patch-clamp technique. The results indicated that: (1) the amplitudes of transient outward potassium current (I A) and delayed rectifier potassium current (I K) were significantly decreased by 10 -7g/ml nano-WC, while the current-voltage curves of I A and I K were significantly decreased by nano-WC from +10 to +90mV. (2) Nano-WC produced a depolarizing shift in the steady-state activation curve of I A and I K with increased slope factors, and delayed the recovery of I A from inactivation, but no significant effects were found on the inactivation of I A. (3) Nano-WC prolonged the evoked action potential duration and lowered the firing rate. These results suggest that 10 -7g/ml nano-WC can decrease the amplitudes of I A and I K currents by reducing the opening number of voltage-gated potassium channels and delaying the recovery of I A from inactivation, which indicate that nano-WC has the potential neurotoxicity.",

keywords = "Delayed rectifier potassium current, Hippocampus, Neurotoxicity, Transient outward potassium current, Tungsten carbide nanoparticles",

author = "Dehong Shan and Yongling Xie and Guogang Ren and Zhuo Yang",

year = "2012",

month = mar,

day = "7",

doi = "10.1016/j.toxlet.2011.12.001",

language = "English",

volume = "209",

pages = "129--135",

journal = "Toxicology Letters",

issn = "0378-4274",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Inhibitory effect of tungsten carbide nanoparticles on voltage-gated potassium currents of hippocampal CA1 neurons

AU - Shan, Dehong

AU - Xie, Yongling

AU - Ren, Guogang

AU - Yang, Zhuo

PY - 2012/3/7

Y1 - 2012/3/7

N2 - The effects of tungsten carbide nanoparticles (nano-WC) on the properties of voltage-dependent potassium currents and evoked action potentials were studied in the hippocampal CA1 pyramidal neurons of rats at the ages of postnatal days 10-14 using the whole-cell patch-clamp technique. The results indicated that: (1) the amplitudes of transient outward potassium current (I A) and delayed rectifier potassium current (I K) were significantly decreased by 10 -7g/ml nano-WC, while the current-voltage curves of I A and I K were significantly decreased by nano-WC from +10 to +90mV. (2) Nano-WC produced a depolarizing shift in the steady-state activation curve of I A and I K with increased slope factors, and delayed the recovery of I A from inactivation, but no significant effects were found on the inactivation of I A. (3) Nano-WC prolonged the evoked action potential duration and lowered the firing rate. These results suggest that 10 -7g/ml nano-WC can decrease the amplitudes of I A and I K currents by reducing the opening number of voltage-gated potassium channels and delaying the recovery of I A from inactivation, which indicate that nano-WC has the potential neurotoxicity.

AB - The effects of tungsten carbide nanoparticles (nano-WC) on the properties of voltage-dependent potassium currents and evoked action potentials were studied in the hippocampal CA1 pyramidal neurons of rats at the ages of postnatal days 10-14 using the whole-cell patch-clamp technique. The results indicated that: (1) the amplitudes of transient outward potassium current (I A) and delayed rectifier potassium current (I K) were significantly decreased by 10 -7g/ml nano-WC, while the current-voltage curves of I A and I K were significantly decreased by nano-WC from +10 to +90mV. (2) Nano-WC produced a depolarizing shift in the steady-state activation curve of I A and I K with increased slope factors, and delayed the recovery of I A from inactivation, but no significant effects were found on the inactivation of I A. (3) Nano-WC prolonged the evoked action potential duration and lowered the firing rate. These results suggest that 10 -7g/ml nano-WC can decrease the amplitudes of I A and I K currents by reducing the opening number of voltage-gated potassium channels and delaying the recovery of I A from inactivation, which indicate that nano-WC has the potential neurotoxicity.

KW - Delayed rectifier potassium current

KW - Hippocampus

KW - Neurotoxicity

KW - Transient outward potassium current

KW - Tungsten carbide nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=84862812425&partnerID=8YFLogxK

U2 - 10.1016/j.toxlet.2011.12.001

DO - 10.1016/j.toxlet.2011.12.001

M3 - Article

C2 - 22192952

AN - SCOPUS:84862812425

SN - 0378-4274

VL - 209

SP - 129

EP - 135

JO - Toxicology Letters

JF - Toxicology Letters

IS - 2

ER -

Inhibitory effect of tungsten carbide nanoparticles on voltage-gated potassium currents of hippocampal CA1 neurons

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this