Abstract
Isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (CMV). Virus instability before, during and after inoculation of the protoplasts resulted in low infectivities when extracts were assayed on cowpea; however, viral RNA extraction improved the bioassay technique. Attempts to optimize inoculation and incubation of protoplasts are outlined, incorporating the improved assay.
Original language | English |
---|---|
Pages (from-to) | 307-313 |
Number of pages | 7 |
Journal | Archives of Virology |
Volume | 52 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1976 |