Investigations on the infection of cucumber mesophyll protoplasts with cucumber mosaic virus

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Abstract

Isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (CMV). Virus instability before, during and after inoculation of the protoplasts resulted in low infectivities when extracts were assayed on cowpea; however, viral RNA extraction improved the bioassay technique. Attempts to optimize inoculation and incubation of protoplasts are outlined, incorporating the improved assay.
Original languageEnglish
Pages (from-to)307-313
Number of pages7
JournalArchives of Virology
Volume52
Issue number4
DOIs
Publication statusPublished - 1976

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