Isolation and characterization of temperate bacteriophages of Clostridium difficile

Shan Goh; Thomas V Riley; Barbara J Chang

doi:10.1128/AEM.71.2.1079-1083.2005

Isolation and characterization of temperate bacteriophages of Clostridium difficile

Shan Goh, Thomas V Riley, Barbara J Chang

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.

Original language	English
Pages (from-to)	1079-83
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	2
DOIs	https://doi.org/10.1128/AEM.71.2.1079-1083.2005
Publication status	Published - Feb 2005

Keywords

Animals
Bacteriophages
Clostridium difficile
DNA, Viral
Enterocolitis, Pseudomembranous
Humans
Lysogeny
Molecular Sequence Data
Sequence Analysis, DNA
Virus Activation
Journal Article

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10.1128/AEM.71.2.1079-1083.2005

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abstract = "The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.",

keywords = "Animals, Bacteriophages, Clostridium difficile, DNA, Viral, Enterocolitis, Pseudomembranous, Humans, Lysogeny, Molecular Sequence Data, Sequence Analysis, DNA, Virus Activation, Journal Article",

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AU - Riley, Thomas V

AU - Chang, Barbara J

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AB - The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.

KW - Animals

KW - Bacteriophages

KW - Clostridium difficile

KW - DNA, Viral

KW - Enterocolitis, Pseudomembranous

KW - Humans

KW - Lysogeny

KW - Molecular Sequence Data

KW - Sequence Analysis, DNA

KW - Virus Activation

KW - Journal Article

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DO - 10.1128/AEM.71.2.1079-1083.2005

M3 - Article

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VL - 71

SP - 1079

EP - 1083

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 2

ER -

Isolation and characterization of temperate bacteriophages of Clostridium difficile

Abstract

Keywords

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