Junction sequences generated by one-ended transposition

S. Motsch; R. Schmitt; P. Avila; F. de la Cruz; Elaine Ward; J. Grinsted

doi:10.1093/nar/13.9.3335

Junction sequences generated by one-ended transposition

S. Motsch, R. Schmitt, P. Avila, F. de la Cruz, Elaine Ward, J. Grinsted

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22 Citations (Scopus)

Abstract

In the presence of the cognate transposase. plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids (‘one-ended transposition’) (1, 2). The junctions across the sequences of donor and recipient DNA In recombinants generated by this process have been determined. These show that the segment of donor DNA starts precisely at the IR sequence (It is variable at the other end), and is flanked by a direct repeat of host DNA (usually 5bp) that was present only once in the original host sequence. These are characteristics of recombinants generated by transposition of Tn21 and Tnl721 themselves. suggesting that the mechanism of one-ended transposition is very simiiar to that of the corresponding entire elements

Original language	English
Pages (from-to)	3335-3342
Number of pages	8
Journal	Nucleic Acids Research
Volume	13
Issue number	9
DOIs	https://doi.org/10.1093/nar/13.9.3335
Publication status	Published - May 1985

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title = "Junction sequences generated by one-ended transposition",

abstract = "In the presence of the cognate transposase. plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids ({\textquoteleft}one-ended transposition{\textquoteright}) (1, 2). The junctions across the sequences of donor and recipient DNA In recombinants generated by this process have been determined. These show that the segment of donor DNA starts precisely at the IR sequence (It is variable at the other end), and is flanked by a direct repeat of host DNA (usually 5bp) that was present only once in the original host sequence. These are characteristics of recombinants generated by transposition of Tn21 and Tnl721 themselves. suggesting that the mechanism of one-ended transposition is very simiiar to that of the corresponding entire elements",

author = "S. Motsch and R. Schmitt and P. Avila and {de la Cruz}, F. and Elaine Ward and J. Grinsted",

year = "1985",

month = may,

doi = "10.1093/nar/13.9.3335",

language = "English",

volume = "13",

pages = "3335--3342",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press (OUP)",

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TY - JOUR

T1 - Junction sequences generated by one-ended transposition

AU - Motsch, S.

AU - Schmitt, R.

AU - Avila, P.

AU - de la Cruz, F.

AU - Ward, Elaine

AU - Grinsted, J.

PY - 1985/5

Y1 - 1985/5

N2 - In the presence of the cognate transposase. plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids (‘one-ended transposition’) (1, 2). The junctions across the sequences of donor and recipient DNA In recombinants generated by this process have been determined. These show that the segment of donor DNA starts precisely at the IR sequence (It is variable at the other end), and is flanked by a direct repeat of host DNA (usually 5bp) that was present only once in the original host sequence. These are characteristics of recombinants generated by transposition of Tn21 and Tnl721 themselves. suggesting that the mechanism of one-ended transposition is very simiiar to that of the corresponding entire elements

AB - In the presence of the cognate transposase. plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids (‘one-ended transposition’) (1, 2). The junctions across the sequences of donor and recipient DNA In recombinants generated by this process have been determined. These show that the segment of donor DNA starts precisely at the IR sequence (It is variable at the other end), and is flanked by a direct repeat of host DNA (usually 5bp) that was present only once in the original host sequence. These are characteristics of recombinants generated by transposition of Tn21 and Tnl721 themselves. suggesting that the mechanism of one-ended transposition is very simiiar to that of the corresponding entire elements

U2 - 10.1093/nar/13.9.3335

DO - 10.1093/nar/13.9.3335

M3 - Article

SN - 0305-1048

VL - 13

SP - 3335

EP - 3342

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 9

ER -

Junction sequences generated by one-ended transposition

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