Mesenchymal stromal cell derived extracellular vesicles reduce hypoxia-ischaemia induced perinatal injury

Claudia Sisa, Sharad Kholia, Jordan Naylor, Maria Beatriz Herrera Sanchez, Stefania Bruno, Maria Chaiara Deregibus, Giovanni Camussi, Jameel Inal, Sigrun Lange, Mariya Hristova

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)
29 Downloads (Pure)


Background: Neonatal hypoxic-ischemic (HI) insult is a leading cause of disability and death in newborns, with therapeutic hypothermia being the only currently available clinical intervention. Thus there is a great need for adjunct and novel treatments for enhanced or alternative post-HI neuroprotection. Extracellular vesicles (EVs) derived from mesenchymal stromal/stem cells (MSCs) have recently been shown to exhibit regenerative effects in various injury models. Here we present findings showing neuroprotective effects of MSC-derived EVs in the Rice-Vannucci model of severe HI-induced neonatal brain insult.

Methods: Mesenchymal stromal/stem cell-derived EVs were applied intranasally immediately post HI-insult and behavioral outcomes were observed 48 h following MSC-EV treatment, as assessed by negative geotaxis. Brains were thereafter excised and assessed for changes in glial responses, cell death, and neuronal loss as markers of damage at 48 h post HI-insult.

Results: Brains of the MSC-EV treated group showed a significant decrease in microglial activation, cell death, and percentage tissue volume loss in multiple brain regions, compared to the control-treated groups. Furthermore, negative geotaxis test showed improved behavioral outcomes at 48 h following MSC-EV treatment.

Conclusion: Our findings highlight the clinical potential of using MSC-derived EVs following neonatal hypoxia-ischaemia.

Original languageEnglish
Article number282
Pages (from-to)282
Number of pages10
JournalFrontiers in Physiology
Issue numberMAR
Publication statusPublished - 19 Mar 2019


  • Mesenchymal Stem Cells
  • Extracellular vesicles (EVs)
  • Perinatal Brain Injury
  • Ischaemia
  • Neuroprotection
  • Extracellular vesicles
  • Hypoxia
  • Mesenchymal stromal/stem cells
  • Microglia


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