Mimicking cascading liver metabolic reactions on a microfluidic platform

G. Kulsharova; P. Panjan; Y. Y. Chiang; N. Dimov; M. P.C. Marques; A. M. Sesay; F. Baganz; N. Szita

Mimicking cascading liver metabolic reactions on a microfluidic platform

G. Kulsharova, P. Panjan, Y. Y. Chiang, N. Dimov, M. P.C. Marques, A. M. Sesay, F. Baganz, N. Szita

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We describe a novel microfluidic electrochemical biosensor chip as a first step towards the development of a microfluidic enzymatic system capable of rapidly analyzing cascading metabolic reactions in the liver. The model enzymatic cascade is based on the essential human metabolism of antimalarial drugs by the cytochrome P450 (P450) enzyme and glucosyltransferase (UGT) enzymes. Here we report the first part of the system: a P450 chip integrated with a novel redox biosensor for monitoring enzyme-substrate binding. This microfluidic platform allows successful enzyme-substrate detection and can predict enzymatic activity in continuous flow.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	539-540
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

Keywords

Electrochemical microfluidic platform
Mimicking liver metabolism
P450 chip

Cite this

@inproceedings{2002583841434917bee1ffb8c7873caa,

title = "Mimicking cascading liver metabolic reactions on a microfluidic platform",

abstract = "We describe a novel microfluidic electrochemical biosensor chip as a first step towards the development of a microfluidic enzymatic system capable of rapidly analyzing cascading metabolic reactions in the liver. The model enzymatic cascade is based on the essential human metabolism of antimalarial drugs by the cytochrome P450 (P450) enzyme and glucosyltransferase (UGT) enzymes. Here we report the first part of the system: a P450 chip integrated with a novel redox biosensor for monitoring enzyme-substrate binding. This microfluidic platform allows successful enzyme-substrate detection and can predict enzymatic activity in continuous flow.",

keywords = "Electrochemical microfluidic platform, Mimicking liver metabolism, P450 chip",

author = "G. Kulsharova and P. Panjan and Chiang, {Y. Y.} and N. Dimov and Marques, {M. P.C.} and Sesay, {A. M.} and F. Baganz and N. Szita",

year = "2016",

language = "English",

pages = "539--540",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Kulsharova, G, Panjan, P, Chiang, YY, Dimov, N, Marques, MPC, Sesay, AM, Baganz, F & Szita, N 2016, Mimicking cascading liver metabolic reactions on a microfluidic platform. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 539-540, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

Mimicking cascading liver metabolic reactions on a microfluidic platform. / Kulsharova, G.; Panjan, P.; Chiang, Y. Y. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 539-540.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Mimicking cascading liver metabolic reactions on a microfluidic platform

AU - Kulsharova, G.

AU - Panjan, P.

AU - Chiang, Y. Y.

AU - Dimov, N.

AU - Marques, M. P.C.

AU - Sesay, A. M.

AU - Baganz, F.

AU - Szita, N.

PY - 2016

Y1 - 2016

N2 - We describe a novel microfluidic electrochemical biosensor chip as a first step towards the development of a microfluidic enzymatic system capable of rapidly analyzing cascading metabolic reactions in the liver. The model enzymatic cascade is based on the essential human metabolism of antimalarial drugs by the cytochrome P450 (P450) enzyme and glucosyltransferase (UGT) enzymes. Here we report the first part of the system: a P450 chip integrated with a novel redox biosensor for monitoring enzyme-substrate binding. This microfluidic platform allows successful enzyme-substrate detection and can predict enzymatic activity in continuous flow.

AB - We describe a novel microfluidic electrochemical biosensor chip as a first step towards the development of a microfluidic enzymatic system capable of rapidly analyzing cascading metabolic reactions in the liver. The model enzymatic cascade is based on the essential human metabolism of antimalarial drugs by the cytochrome P450 (P450) enzyme and glucosyltransferase (UGT) enzymes. Here we report the first part of the system: a P450 chip integrated with a novel redox biosensor for monitoring enzyme-substrate binding. This microfluidic platform allows successful enzyme-substrate detection and can predict enzymatic activity in continuous flow.

KW - Electrochemical microfluidic platform

KW - Mimicking liver metabolism

KW - P450 chip

UR - http://www.scopus.com/inward/record.url?scp=85014183049&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85014183049

SP - 539

EP - 540

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Mimicking cascading liver metabolic reactions on a microfluidic platform

Abstract

Conference

Keywords

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