Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Joshua A. Welsh; Deborah C. I. Goberdhan; Lorraine O'Driscoll; Edit I. Buzas; Cherie Blenkiron; Benedetta Bussolati; Houjian Cai; Dolores Di Vizio; Tom A. P. Driedonks; Uta Erdbrügger; Juan M. Falcon-Perez; Qing-Ling Fu; Andrew F. Hill; Metka Lenassi; Sai Kiang Lim; Mỹ G. Mahoney; Sujata Mohanty; Andreas Möller; Rienk Nieuwland; Takahiro Ochiya; Susmita Sahoo; Ana C. Torrecilhas; Lei Zheng; Andries Zijlstra; Sarah Abuelreich; Reem Bagabas; Paolo Bergese; Esther M. Bridges; Marco Brucale; Dylan Burger; Randy P. Carney; Emanuele Cocucci; Rossella Crescitelli; Edveena Hanser; Adrian L. Harris; Norman J. Haughey; An Hendrix; Alexander R. Ivanov; Tijana Jovanovic-Talisman; Nicole A. Kruh-Garcia; Vroniqa Ku'ulei-Lyn Faustino; Diego Kyburz; Cecilia Lässer; Kathleen M. Lennon; Jan Lötvall; Adam L. Maddox; Elena S. Martens-Uzunova; Rachel R. Mizenko; Lauren A. Newman; Andrea Ridolfi; Eva Rohde; Tatu Rojalin; Andrew Rowland; Andras Saftics; Ursula S. Sandau; Julie A. Saugstad; Faezeh Shekari; Simon Swift; Dmitry Ter-Ovanesyan; Juan P. Tosar; Zivile Useckaite; Francesco Valle; Zoltan Varga; Edwin van der Pol; Martijn J. C. van Herwijnen; Marca H. M. Wauben; Ann M. Wehman; Sarah Williams; Andrea Zendrini; Alan J. Zimmerman; Clotilde Théry; Kenneth W. Witwer; MISEV Consortium; Jameel Inal

doi:10.1002/jev2.12404

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrügger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro OchiyaSusmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic-Talisman, Nicole A. Kruh-Garcia, Vroniqa Ku'ulei-Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens-Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter-Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J. C. van Herwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, Clotilde Théry, Kenneth W. Witwer, MISEV Consortium, Jameel Inal

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Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Original language	English
Article number	12404
Pages (from-to)	1-84
Number of pages	84
Journal	Journal of Extracellular Vesicles
Volume	13
Issue number	2
DOIs	https://doi.org/10.1002/jev2.12404
Publication status	Published - 7 Feb 2024

Keywords

ectosomes
exosomes
extracellular vesicles
extracellular particles
guidelines
microparticles
microvesicles
minimal information requirements
MISEV
reproducibility
rigor
standardisation

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© 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/
Final published version, 2.76 MBLicence: CC BY

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Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., Falcon-Perez, J. M., Fu, Q.-L., Hill, A. F., Lenassi, M., Lim, S. K., Mahoney, M. G., Mohanty, S., Möller, A., Nieuwland, R., ... Inal, J. (2024). Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles, 13(2), 1-84. Article 12404. https://doi.org/10.1002/jev2.12404

@article{76cf774eae4849a9ba0ea62fdc63960a,

title = "Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches",

abstract = "Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.",

keywords = "ectosomes, exosomes, extracellular vesicles, extracellular particles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation",

author = "Welsh, {Joshua A.} and Goberdhan, {Deborah C. I.} and Lorraine O'Driscoll and Buzas, {Edit I.} and Cherie Blenkiron and Benedetta Bussolati and Houjian Cai and {Di Vizio}, Dolores and Driedonks, {Tom A. P.} and Uta Erdbr{\"u}gger and Falcon-Perez, {Juan M.} and Qing-Ling Fu and Hill, {Andrew F.} and Metka Lenassi and Lim, {Sai Kiang} and Mahoney, {Mỹ G.} and Sujata Mohanty and Andreas M{\"o}ller and Rienk Nieuwland and Takahiro Ochiya and Susmita Sahoo and Torrecilhas, {Ana C.} and Lei Zheng and Andries Zijlstra and Sarah Abuelreich and Reem Bagabas and Paolo Bergese and Bridges, {Esther M.} and Marco Brucale and Dylan Burger and Carney, {Randy P.} and Emanuele Cocucci and Rossella Crescitelli and Edveena Hanser and Harris, {Adrian L.} and Haughey, {Norman J.} and An Hendrix and Ivanov, {Alexander R.} and Tijana Jovanovic-Talisman and Kruh-Garcia, {Nicole A.} and {Ku'ulei-Lyn Faustino}, Vroniqa and Diego Kyburz and Cecilia L{\"a}sser and Lennon, {Kathleen M.} and Jan L{\"o}tvall and Maddox, {Adam L.} and Martens-Uzunova, {Elena S.} and Mizenko, {Rachel R.} and Newman, {Lauren A.} and Andrea Ridolfi and Eva Rohde and Tatu Rojalin and Andrew Rowland and Andras Saftics and Sandau, {Ursula S.} and Saugstad, {Julie A.} and Faezeh Shekari and Simon Swift and Dmitry Ter-Ovanesyan and Tosar, {Juan P.} and Zivile Useckaite and Francesco Valle and Zoltan Varga and {van der Pol}, Edwin and {van Herwijnen}, {Martijn J. C.} and Wauben, {Marca H. M.} and Wehman, {Ann M.} and Sarah Williams and Andrea Zendrini and Zimmerman, {Alan J.} and Clotilde Th{\'e}ry and Witwer, {Kenneth W.} and MISEV Consortium and Jameel Inal",

note = "{\textcopyright} 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/",

year = "2024",

month = feb,

day = "7",

doi = "10.1002/jev2.12404",

language = "English",

volume = "13",

pages = "1--84",

journal = "Journal of Extracellular Vesicles",

publisher = "Taylor & Francis Group",

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Welsh, JA, Goberdhan, DCI, O'Driscoll, L, Buzas, EI, Blenkiron, C, Bussolati, B, Cai, H, Di Vizio, D, Driedonks, TAP, Erdbrügger, U, Falcon-Perez, JM, Fu, Q-L, Hill, AF, Lenassi, M, Lim, SK, Mahoney, MG, Mohanty, S, Möller, A, Nieuwland, R, Ochiya, T, Sahoo, S, Torrecilhas, AC, Zheng, L, Zijlstra, A, Abuelreich, S, Bagabas, R, Bergese, P, Bridges, EM, Brucale, M, Burger, D, Carney, RP, Cocucci, E, Crescitelli, R, Hanser, E, Harris, AL, Haughey, NJ, Hendrix, A, Ivanov, AR, Jovanovic-Talisman, T, Kruh-Garcia, NA, Ku'ulei-Lyn Faustino, V, Kyburz, D, Lässer, C, Lennon, KM, Lötvall, J, Maddox, AL, Martens-Uzunova, ES, Mizenko, RR, Newman, LA, Ridolfi, A, Rohde, E, Rojalin, T, Rowland, A, Saftics, A, Sandau, US, Saugstad, JA, Shekari, F, Swift, S, Ter-Ovanesyan, D, Tosar, JP, Useckaite, Z, Valle, F, Varga, Z, van der Pol, E, van Herwijnen, MJC, Wauben, MHM, Wehman, AM, Williams, S, Zendrini, A, Zimmerman, AJ, Théry, C, Witwer, KW, Consortium, MISEV & Inal, J 2024, 'Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches', Journal of Extracellular Vesicles, vol. 13, no. 2, 12404, pp. 1-84. https://doi.org/10.1002/jev2.12404

TY - JOUR

T1 - Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

AU - Welsh, Joshua A.

AU - Goberdhan, Deborah C. I.

AU - O'Driscoll, Lorraine

AU - Buzas, Edit I.

AU - Blenkiron, Cherie

AU - Bussolati, Benedetta

AU - Cai, Houjian

AU - Di Vizio, Dolores

AU - Driedonks, Tom A. P.

AU - Erdbrügger, Uta

AU - Falcon-Perez, Juan M.

AU - Fu, Qing-Ling

AU - Hill, Andrew F.

AU - Lenassi, Metka

AU - Lim, Sai Kiang

AU - Mahoney, Mỹ G.

AU - Mohanty, Sujata

AU - Möller, Andreas

AU - Nieuwland, Rienk

AU - Ochiya, Takahiro

AU - Sahoo, Susmita

AU - Torrecilhas, Ana C.

AU - Zheng, Lei

AU - Zijlstra, Andries

AU - Abuelreich, Sarah

AU - Bagabas, Reem

AU - Bergese, Paolo

AU - Bridges, Esther M.

AU - Brucale, Marco

AU - Burger, Dylan

AU - Carney, Randy P.

AU - Cocucci, Emanuele

AU - Crescitelli, Rossella

AU - Hanser, Edveena

AU - Harris, Adrian L.

AU - Haughey, Norman J.

AU - Hendrix, An

AU - Ivanov, Alexander R.

AU - Jovanovic-Talisman, Tijana

AU - Kruh-Garcia, Nicole A.

AU - Ku'ulei-Lyn Faustino, Vroniqa

AU - Kyburz, Diego

AU - Lässer, Cecilia

AU - Lennon, Kathleen M.

AU - Lötvall, Jan

AU - Maddox, Adam L.

AU - Martens-Uzunova, Elena S.

AU - Mizenko, Rachel R.

AU - Newman, Lauren A.

AU - Ridolfi, Andrea

AU - Rohde, Eva

AU - Rojalin, Tatu

AU - Rowland, Andrew

AU - Saftics, Andras

AU - Sandau, Ursula S.

AU - Saugstad, Julie A.

AU - Shekari, Faezeh

AU - Swift, Simon

AU - Ter-Ovanesyan, Dmitry

AU - Tosar, Juan P.

AU - Useckaite, Zivile

AU - Valle, Francesco

AU - Varga, Zoltan

AU - van der Pol, Edwin

AU - van Herwijnen, Martijn J. C.

AU - Wauben, Marca H. M.

AU - Wehman, Ann M.

AU - Williams, Sarah

AU - Zendrini, Andrea

AU - Zimmerman, Alan J.

AU - Théry, Clotilde

AU - Witwer, Kenneth W.

AU - Consortium, MISEV

AU - Inal, Jameel

N1 - © 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/

PY - 2024/2/7

Y1 - 2024/2/7

N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

AB - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

KW - ectosomes

KW - exosomes

KW - extracellular vesicles

KW - extracellular particles

KW - guidelines

KW - microparticles

KW - microvesicles

KW - minimal information requirements

KW - MISEV

KW - reproducibility

KW - rigor

KW - standardisation

U2 - 10.1002/jev2.12404

DO - 10.1002/jev2.12404

M3 - Article

VL - 13

SP - 1

EP - 84

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

IS - 2

M1 - 12404

ER -

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

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Keywords

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